Development of EST-SSRs in Cucumis sativus from sequence database
Development of EST-SSRs in Cucumis sativus from sequence database
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DOI：
10.1111/j.1471-8286.2006.01500.x
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发表时间：
2006-12-01
期刊：
影响因子：
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通讯作者：
Yu, Z.
中科院分区：
文献类型：
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作者：
Kong, Q.;Xiang, C.;Yu, Z.
基于表达序列标签的简单重复序列标记（EST-SSR）在植物育种和种质资源保存中具有重要的应用价值，近年来，这类标记的开发受到越来越多的关注。从公共序列数据库中的黄瓜EST序列中筛选出20个多态性SSR标记。在20个黄瓜基因型中，平均每个位点的等位基因数为3.3个，等位基因数在2 ~ 6个之间，平均期望杂合度为0.477。13对引物在梅洛中也检测到扩增产物。这些信息丰富的EST-SSR标记可用于黄瓜遗传改良。
Simple sequence repeat markers derived from expressed sequence tags (EST-SSR) are potentially valuable tools for plant breeding and germplasm collection conservation, and increasingly, efforts have been made for developing this type of marker. We have identified 20 polymorphic SSR markers from cucumber ESTs deposited in public sequence database. The average allele number was 3.3 per locus, ranging from two to six alleles during screening 20 cucumber genotypes with the mean expected heterozygosity of 0.477. Amplification products were also detected by 13 pairs of primer in Cucumis melo. These informative EST-SSR markers can be used in cucumber genetic improvement projects.