Ectomycorrhizal fungal community in alkaline-saline soil in northeastern China
Ectomycorrhizal fungal community in alkaline-saline soil in northeastern China
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DOI：
10.1007/s00572-008-0219-9
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发表时间：
2009-06-01
期刊：
影响因子：
3.9
通讯作者：
Liu, Shenkui
中科院分区：
文献类型：
--
作者：
Ishida, Takahide A.;Nara, Kazuhide;Liu, Shenkui
盐碱土广泛分布于世界干旱和半干旱地区，造成严重的环境和农业问题。为了进一步了解外生菌根真菌（EMF）对盐碱土的适应性，对生长在中国东北盐碱土（pH值高达9.2）上的蒙古杨柳（Salix lineistipularis）上的EMF群落进行了研究。总共，75根样品收集25个杨柳个人超过4.7公顷。为了鉴定外生菌根根尖中的真菌种类，我们使用了核糖体DNA的内部转录间隔区的末端限制性片段长度多态性和测序分析。我们检测到11种EMF，包括担子菌门的Inocybe，Hebeloma和Tomentella，以及三种子囊菌。使用主要估计器估计研究部位的EMF丰富度为15-17。最丰富的物种是Geopora sp. 1，而没有Geopora占主导地位的EMF社区已报告到目前为止。系统发育分析表明，包括Geopora sp. 1在内的多个类群主要分布在碱性土壤生境中，表明其对高土壤pH的适应性。由于EMF是宿主植物生长所不可或缺的，本研究中检测到的EMF物种可能对盐碱地的恢复有用。
Alkaline-saline soil is widespread in arid and semiarid regions of the world and causes severe environmental and agricultural problems. To advance our understanding of the adaptation of ectomycorrhizal fungi (EMF) to alkaline-saline soil, we investigated EMF communities on Mongolian willow (Salix linearistipularis) growing in alkaline-saline soil (up to pH 9.2) in northeastern China. In total, 75 root samples were collected from 25 willow individuals over 4.7 ha. To identify fungal species in ectomycorrhizal root tips, we used terminal restriction fragment length polymorphism and sequencing analyses of the internal transcribed spacer region of ribosomal DNA. We detected 11 EMF species, including species of Inocybe, Hebeloma, and Tomentella of the Basidiomycota and three Ascomycota species. The EMF richness of the study site was estimated to be 15-17 using major estimators. The most abundant species was Geopora sp. 1, while no Geopora-dominated EMF communities have been reported so far. Phylogenetic analysis showed that the phylogroup including Geopora sp. 1 has been found mostly in alkaline soil habitats, indicating its adaptation to high soil pH. Because EMF are indispensable for host plant growth, the EMF species detected in this study may be useful for restoration of alkaline-saline areas.