Molecular investigation and phylogeny of Anaplasmataceae species infecting domestic animals and ticks in Corsica, France.
Molecular investigation and phylogeny of Anaplasmataceae species infecting domestic animals and ticks in Corsica, France.
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DOI：
10.1186/s13071-017-2233-2
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发表时间：
2017-06-23
影响因子：
3.2
通讯作者：
Mediannikov O
中科院分区：
文献类型：
--
作者：
Dahmani M;Davoust B;Tahir D;Raoult D;Fenollar F;Mediannikov O
科西嘉岛是位于地中海的法国岛屿。该岛提供了适宜的自然条件来研究疾病生态学，特别是蜱传疾病和动物和蜱的新发疾病。无形体科是立克次体目的一个成员；它包括无原体属、埃利希氏体属、新立克次体属和沃尔巴克氏体属。无形体病和埃利希体病传统上是指由细胞内无形体和埃利希体属专性细菌引起的疾病。本研究的目的是鉴定和估计无形体科物种在科西嘉岛家畜和蜱的流行情况。本研究在科西嘉岛收集了458份羊、牛、马、山羊、狗的血液样本和123份牛的蜱虫样本。基于23S rRNA、rpoB和groEl基因对无浆菌科细菌进行实时荧光定量PCR筛选和遗传鉴定。本研究收集到2种蜱：囊鼻蜱（118种）和边缘玻眼蜱（5种）。分子检测结果显示，32.1%（147/458）的血液样本呈无形体菌感染阳性。42.3%（93/220）的绵羊检出卵巢无原体。从100%（12/12）的牛和2只（2/123）的法氏乳杆菌中扩增出边缘无原体。从17.3%（38/220）的绵羊和80%（4/5）的山羊中分别扩增出了ovis、corsicananum、mediterraneum和marginale-like无原体。最后，一只法氏蜱被发现含有犬伊氏蜱的DNA。所有马和狗的样本均未检测到无形体科感染。据我们所知，本研究是首次对科西嘉岛动物和蜱感染无形体科物种进行流行病学调查，有助于科西嘉岛流行无形体科物种的鉴定。
Corsica is a French island situated in the Mediterranean Sea. The island provides suitable natural conditions to study disease ecology, especially tick-borne diseases and emerging diseases in animals and ticks. The family Anaplasmataceae is a member of the order Rickettsiales; it includes the genera Anaplasma, Ehrlichia, Neorickettsia and Wolbachia. Anaplasmosis and ehrlichiosis traditionally refer to diseases caused by obligate intracellular bacteria of the genera Anaplasma and Ehrlichia. The aim of this study was to identify and estimate the prevalence of Anaplasmataceae species infecting domestic animals and ticks in Corsica. In this study, 458 blood samples from sheep, cattle, horses, goats, dogs, and 123 ticks removed from cattle, were collected in Corsica. Quantitative real-time PCR screening and genetic characterisation of Anaplasmataceae bacteria were based on the 23S rRNA, rpoB and groEl genes. Two tick species were collected in the present study: Rhipicephalus bursa (118) and Hyalomma marginatum marginatum (5). Molecular investigation showed that 32.1% (147/458) of blood samples were positive for Anaplasmataceae infection. Anaplasma ovis was identified in 42.3% (93/220) of sheep. Anaplasma marginale was amplified from 100% (12/12) of cattle and two R. bursa (2/123). Several potentially new species were also identified: Anaplasma cf. ovis, “Candidatus Anaplasma corsicanum”, “Candidatus Anaplasma mediterraneum” were amplified from 17.3% (38/220) of sheep, and Anaplasma sp. marginale-like was amplified from 80% (4/5) of goats. Finally, one R. bursa tick was found to harbour the DNA of E. canis. All samples from horses and dogs were negative for Anaplasmataceae infection. To our knowledge, this study is the first epidemiological survey on Anaplasmataceae species infecting animals and ticks in Corsica and contributes toward the identification of current Anaplasmataceae species circulating in Corsica.