Differences in non-enzymatic glycation and collagen cross-links between human cortical and cancellous bone.
Differences in non-enzymatic glycation and collagen cross-links between human cortical and cancellous bone.
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DOI：
10.1007/s00198-013-2319-4
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发表时间：
2013-09
期刊：
影响因子：
--
通讯作者：
Vashishth D
中科院分区：
文献类型：
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作者：
Karim L;Tang SY;Sroga GE;Vashishth D
由非酶糖化产生的胶原交联（晚期糖化终产物[AGEs]）的积累使骨的机械性能和抗骨折性恶化。虽然一个单一的AGE，戊糖苷，通常被用作一个代表性的标志物，目前还不清楚它是否定量反映总荧光AGEs在骨。本研究的目的是建立松质骨和皮质骨中戊糖苷和总AGEs之间的关系。在170个人骨样本中定量测定了Pentosidine和总AGEs。总荧光AGEs测定在28个额外的松质骨和皮质骨标本相同的表观体积，培养在控制或体外糖化溶液。戊糖和总AGEs之间的相关性和皮质骨和松质骨组之间的差异进行了测定。Pentosidine与松质骨中的总AGEs相关（r=0.53，p<0.0001），与皮质骨中的AGEs弱相关（r=0.23，p<0.05）。松质骨中戊糖甙和总AGEs含量均高于皮质骨（p<0.001）。体外糖基化亚组研究显示松质骨比皮质骨积累更多的AGEs（p<0.05）。pentosidine和总AGEs和他们的积累幅度之间的关系不同的松质骨和皮质骨相同的表观体积，并依赖于每个样品的表面体积比。将骨类型视为两个独立的实体是很重要的，并且除了戊糖苷外，量化总AGEs以允许更全面地分析骨中非酶糖化的影响也是至关重要的。
Accumulation of collagen crosslinks (advanced glycation end products [AGEs]) produced by non-enzymatic glycation deteriorates bone's mechanical properties and fracture resistance. Although a single AGE, pentosidine, is commonly used as a representative marker, it is unclear whether it quantitatively reflects total fluorescent AGEs in bone. The goal of this study was to establish the relationship between pentosidine and total AGEs in cancellous and cortical bone. Pentosidine and total AGEs were quantified in 170 human bone samples. Total fluorescent AGEs were measured in 28 additional cancellous and cortical bone specimens of the same apparent volume that were incubated in control or in vitro glycation solutions. Correlations between pentosidine and total AGEs and differences between cortical and cancellous groups were determined. Pentosidine was correlated with total AGEs in cancellous bone (r=0.53, p<0.0001) and weakly correlated in cortical bone (r=0.23, p<0.05). There was more pentosidine (p<0.01) and total AGEs (p<0.001) in cancellous than in cortical bone. The in vitro glycation sub-study showed that cancellous bone accumulated more AGEs than cortical bone (p<0.05). The relationship between pentosidine and total AGEs and their magnitude of accumulation differed in cancellous and cortical bone of the same apparent volume, and were dependent on the surface-to-volume ratios of each sample. It is important to consider the bone types as two separate entities, and it is crucial to quantify total AGEs in addition to pentosidine to allow for more comprehensive analysis of the effects of non-enzymatic glycation in bone.