Do ‘cheese factory-specific’ mites (Acari: Astigmata) exist in the cheese-ripening cabinet?
Do ‘cheese factory-specific’ mites (Acari: Astigmata) exist in the cheese-ripening cabinet?
复制标题
奶酪熟化柜中是否存在“奶酪工厂特有”螨虫（螨虫：Astigmata）？
DOI：
10.1007/s10493-022-00725-8
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发表时间：
2022
影响因子：
2.2
通讯作者：
OConnor Barry M.
中科院分区：
文献类型：
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作者：
Shimano Satoshi;Hiruta Shimpei F.;Shimizu Nobuhiro;Hagino Wataru;Aoki Jun-ichi;OConnor Barry M.
为了确定传统奶酪成熟过程中使用的螨是否是奶酪工厂的遗传独特性，我们调查了三种类型的传统奶酪中的螨，在成熟过程中使用螨：来自德国的“Würchwitzer Milbenkäse”和来自法国的“Mimolette”和“Artisou”。此外，从法国（Mimolette）和日本（法国的“Laguiole”）的奶酪专卖店以及法国临时市场（“Salers”和“Cantal vieux”）的商店购买传统成熟奶酪，并在本研究中分析了从这些奶酪中获得的螨。从法国和日本的3家传统奶酪生产商和2家奶酪专卖店的成熟柜中分离到的干酪酪螨Tyrolichus casei，其28S rRNA基因的部分序列完全相同。在28S序列测定区域内，所有来自法国临时市场商店的Cantal vieux标本与粗粉螨和固定粉螨的序列完全相同。来自法国临时市场商店的Salers螨个体与Acotyledon paradoxa具有相同的单倍型。对于T。对来自5个不同地区的19个个体进行核基因座分析。通过多重PCR同时扩增超过几千个基因组区域，并且使用MiSeq系统（Illumina）对基因组中被简单序列间重复（ISSR）包围的靶向区域进行测序。从全基因组分析中提取的SNPs表明，来自任何地区的人群中不存在遗传结构。在来自三个地区的五个样本中，这三个地区相距超过500公里，来自完全不同的环境，螨没有地理偏见，但所有螨个体的遗传几乎相同。因此，我们没有发现任何证据支持存在的“奶酪工厂特异性”T。至少在基因分析方面。
To determine whether the mites used in the ripening process of traditional cheeses are genetically unique to cheese factories, we investigated mites from three types of traditional cheeses, that use mites in the ripening process: ‘Würchwitzer Milbenkäse’ from Germany and ‘Mimolette’ and ‘Artisou’ from France. In addition, traditional ripened cheeses were purchased from cheese specialty stores in France (Mimolette) and Japan (‘Laguiole’ from France) as well as stores in temporary markets in France (‘Salers’ and ‘Cantal vieux’) and the mites obtained from those cheeses were analyzed in this study. Partial sequences of the 28S rRNA gene (28S) were determined and used to reconstruct a phylogenetic tree.Tyrolichus casei, the dominant cheese mite species from the ripening cabinets of three traditional cheese producers and two cheese specialty stores in France and Japan, had identical partial 28S sequences. All specimens from Cantal vieux from a store in the temporary market in France had an identical sequence withAcarus siroandAcarus immobilisin the determined region of the 28S sequences. Mite individuals from Salers from a store in the temporary markets in France shared the same haplotype asAcotyledon paradoxa. For theT. caseiindividuals from five different localities (19 individuals in total), the nuclear loci were obtained using MIG-seq. More than several thousand genomic regions are amplified simultaneously by multiplex PCR, and targeting regions surrounded by inter-simple sequence repeats (ISSRs) in the genome were sequenced using the MiSeq system (Illumina). SNPs extracted from this genome-wide analysis showed that no genetic structure existed in the populations from any region. Among the five samples from the three regions, which were more than 500 km apart and from completely different environments, the mites had no geographic bias, but all mite individuals were genetically nearly identical. Thus, we found no evidence to support the existence of ‘cheese factory-specific’T. caseimites, at least in terms of genetic analysis.