In vitro analyses of neurite outgrowth indicate a potential role for tenascin-like molecules in the development of insect olfactory glomeruli.
In vitro analyses of neurite outgrowth indicate a potential role for tenascin-like molecules in the development of insect olfactory glomeruli.
复制标题
神经突生长的体外分析表明腱蛋白样分子在昆虫嗅觉肾小球发育中的潜在作用。
DOI：
10.1002/neu.480250808
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Tolbert,LP
中科院分区：
文献类型：
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作者：
Krull,CE;Oland,LA;Faissner,A;Schachner,M;Tolbert,LP
tenastin样物质与Manduca sexta的嗅叶（触角）中形成肾小球单位周围的胶质细胞有关，并在肾小球形成的关键阶段存在（Krull et al., 1994， J. Neurobiol. 25515434）。成熟叶tenascin样免疫反应性下降，与肾小球内突触形成波和肾小球稳定一致。与神经柱胶质相关的tenastin样分子处于正确的位置，通过将其限制在肾小球来影响生长的神经突的分支模式。在这项研究中，我们观察了培养的飞蛾触角叶神经元对小鼠中枢神经系统腱素的反应。均匀的腱蛋白为细胞体附着和神经突生长提供了较差的底物。神经元细胞体具有由腱蛋白和豆豆蛋白a (con-A)/层粘连蛋白组成的条纹基底，优先附着于con-A/层粘连蛋白通道。大多数神经元在培养的早期和后期都将其分支限制在con- a /层粘连蛋白通道上，但其他神经元则以明显不加区分的方式将过程发送到多个腱状蛋白和con /层粘连蛋白通道上。Tenascin可以抑制大多数触角叶神经元的神经突生长，这提出了与神经柱胶质相关的Tenascin样分子在体内抑制神经突生长到肾小球的可能性。因此，神经胶质细胞与嗅觉轴突协同作用，可能促进了触角叶神经元的肾小球分支模式。o 1994年约翰威利父子公司
Tenascin-like material is associated with glial cells that form borders around developing glomerular units in the olfactory (antennal) lobe of the moth Manduca sexta and is present at critical stages of glomerulus formation (Krull et al., 1994, J. Neurobiol. 25515434). Tenascin-like immunoreactivity declines in the mature lobe, coincident with a wave of synapse formation within the glo-meruli and glomerulus stabilization. Tenascin-like molecules associated with neuropilar glia are in the correct position to influence the branching patterns of growing neurites by constraining them to glomeruli. In this study, we examine the growth of cultured moth antennal-lobe neurons in response to mouse CNS tenascin. Uniform tenascin provides a poor substrate for cell-body attachment and neurite outgrowth. Neuronal cell bodies provided with a striped substratum consisting of tenascin and concanavalin-A (con-A)/laminin attach preferentially to con-A/laminin lanes. Most neurons restrict their branching to con-A/laminin lanes both at early and later times in culture but others send processes across multiple tenascin and con-/laminin lanes in an apparently indiscriminate manner. Tenascin can inhibit the neuritic outgrowth of most antennal-lobe neurons, and this raises the possibility that the tenascin-like molecules associated with neuropilar glia in vivo act to constrain growing neurites to glomeruli. Thus, glial cells, acting in concert with olfactory axons, might act to promote glomerular patterns of branching by antennal-lobe neurons. o 1994 John Wiley & sons, Inc.