Carbon- and Nitrogen-to-Volume Ratios of Bacterioplankton Grown under Different Nutritional Conditions
Carbon- and Nitrogen-to-Volume Ratios of Bacterioplankton Grown under Different Nutritional Conditions
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不同营养条件下生长的浮游细菌的碳氮体积比
DOI：
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发表时间：
1990
影响因子：
4.4
通讯作者：
Yasunori Watanabe
中科院分区：
文献类型：
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作者：
T. Nagata;Yasunori Watanabe
测定了在湖水滤液或富含营养物质（塞斯顿湖的水提取物、葡萄糖、精氨酸、磷酸盐或铵）中生长的淡水细菌组合的碳和氮体积比（C/V和N/V）。通过落射荧光显微照相术测量生物体积，并使用 CHN 分析仪测量碳和氮生物量。尽管营养条每μm3 0.027 pg N [标准偏差；0.011，n = 15]；碳氮重量比，5.6 [标准偏差，2.2，n = 15]）。建议将来自天然和培养细菌组合的平均 C/V 比为每 μm3 0.12 pg C 作为估计淡水细菌生物量的适当转换因子。
Carbon- and nitrogen-to-volume (C/V and N/V) ratios were determined for freshwater bacterial assemblages grown in lake water filtrate or in water enriched with nutrients (aqueous extract of lake seston, glucose, arginine, phosphate, or ammonium). Biovolume was measured by epifluorescence microphotography, and carbon and nitrogen biomasses were measured with a CHN analyzer. Despite large variations of nutritional conditions (i.e., the composition and concentration of the dissolved organic carbon) and different mean cell sizes of the bacterial assemblage (0.17 to 1.8 μm3 per cell), the C/V, N/V, and carbon-to-nitrogen weight ratios varied little (C/V ratio, 0.14 pg of C per μm3 [standard deviation, 0.057; n = 15]; N/V ratio, 0.027 pg of N per μm3 [standard deviation; 0.011, n = 15]; carbon-to-nitrogen weight ratio, 5.6 [standard deviation, 2.2, n = 15]). An average C/V ratio of 0.12 pg of C per μm3 that was derived from natural and cultured bacterial assemblages is proposed as an appropriate conversion factor for estimation of the biomass of freshwater bacteria.