IMMUNOHISTOCHEMICAL AND IMMUNOCYTOCHEMICAL LOCALIZATION OF ATRIAL NATRIURETIC POLYPEPTIDE IN HUMAN ADULT AND FETAL HEARTS
IMMUNOHISTOCHEMICAL AND IMMUNOCYTOCHEMICAL LOCALIZATION OF ATRIAL NATRIURETIC POLYPEPTIDE IN HUMAN ADULT AND FETAL HEARTS
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心房钠尿多肽在成人和胎儿心脏中的免疫组织化学和免疫细胞化学定位
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
A. Kanai
中科院分区：
文献类型：
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作者：
S. Morinaga;M. Tsumuraya;T. Nakajima;Y. Shimosato;K. Yoshinaga;K. Yamaguchi;K. Abe;H. Ohno;A. Kanai
采用抗α-人心房利钠肽（ANP）抗血清免疫过氧化物酶法研究了心房利钠肽（ANP）在成人和胎儿心脏中的定位。光镜下，在心房肌细胞中观察到ANP的反应产物，除了成人心脏的特殊传导系统外，ANP的反应产物被很好地限制在心房和房间隔内。心房各部位免疫染色强度无明显差异。胞浆内ANP免疫反应产物呈细颗粒状，通常集中于核旁肌浆。同时对成人心房ANP进行了电子免疫金染色。在超微结构上，心房特异性颗粒中可见金颗粒。免疫反应性anp在妊娠7、11、12、13、16和17周的所有胎儿心房均有出现。反应产物的分布与成人心脏相似，ANP染色强度随胎龄增加呈增加趋势。
The localization of atrial natriuretic polypeptide (ANP) in human adult and fetal hearts was investigated by an immunoperoxidase procedure using anti-α-human ANP antiserum. Light microscopically, the reaction product for ANP was observed in atrial muscle cells and was well confined within both the atrium and atrial septum with the exception of the specialized conducting system of adult heart. No prominent difference in intensity of immunostaining was observed in any part of the atrium. Intracytoplasmic immunoreaction product for ANP was finely granular and generally concentrated at paranuclear sarcoplasm. Electron immunogold stain for ANP of the human adult atrium was also carried out. Ultrastructurally, gold particles were demonstrated in atrial specific granules. Immunoreactive-ANP was also demonstrated in all atria of fetuses at 7, 11, 12, 13, 16 and 17 weeks of gestation. The distribution of the reaction products was similar to those of the adult heart, and there was a tendency of increased staining intensity for ANP with gestational age.
大鼠肾脏、肾上腺髓质、垂体和心房中心房钠尿因子的免疫细胞化学定位。
DOI：
10.1177/33.8.3160763
发表时间：
1985
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
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作者：
McKenzie,JC;Tanaka,I;Misono,KS;Inagami,T
通讯作者：
Inagami,T