Control of Development, Secondary Metabolism and Light-Dependent Carotenoid Biosynthesis by the Velvet Complex of Neurospora crassa
Control of Development, Secondary Metabolism and Light-Dependent Carotenoid Biosynthesis by the Velvet Complex of Neurospora crassa
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DOI：
10.1534/genetics.119.302277
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发表时间：
2019-07-01
期刊：
影响因子：
3.3
通讯作者：
Bayram, Ozgur
中科院分区：
文献类型：
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作者：
Bayram, Ozlem Sarikaya;Dettmann, Anne;Bayram, Ozgur
粗糙脉孢菌是研究胡萝卜素生物合成和光调节的既定参考生物体。然而，几乎没有证据表明其产生次生代谢产物的能力。在这里，我们报告了真菌特异性调节绒复合物在粗糙脉孢菌发育和次生代谢（SM）中的作用。三种绒蛋白 VE-1、VE-2、VOS-1 和推定的甲基转移酶 LAE-1 显示出光独立的核细胞质定位。在体内发现了两种不同的绒复合物，即异三聚体 VE-1/VE-2/LAE-1 和异二聚体 VE-2/VOS-1。异源三聚体复合物积极调节性发育并抑制无性孢子形成，在缺铁条件下抑制铁载体粪原的产生。 VE-1/VE-2 异二聚体控制胡萝卜素的产生。 VE-1 调节整个基因组>15% 的表达，主要包括调节和发育特征。我们还通过构巢曲霉 veA、velB、laeA、vosA 突变体分别与其粗糙脉孢菌直系同源物 ve-1、ve-2、lae-1 和 vos-1 的互补，研究了天鹅绒复合体的属间功能。构巢曲霉中 VE-1 和 VE-2 的表达通过在体内形成两种功能性嵌合天鹅绒复合物（分别为 VelB/VE-1/LaeA 和 VE-2/VeA/LaeA），成功替代了 VeA 和 VelB 的发育和 SM 功能。相反，veA 的表达恢复了粗糙脉孢菌 ve-1 突变体的表型。所有在构巢曲霉中异源表达的粗糙链孢菌绒蛋白均定位于核部分，与光无关。这些数据强调了粗糙猪笼草和构巢构巢菌复杂结构的保守性。然而，他们也强调了根据不同的生活方式、生态位和个体发育过程，天鹅绒角色的属间相似性和差异。
Neurospora crassa is an established reference organism to investigate carotene biosynthesis and light regulation. However, there is little evidence of its capacity to produce secondary metabolites. Here, we report the role of the fungal-specific regulatory velvet complexes in development and secondary metabolism (SM) in N. crassa. Three velvet proteins VE-1, VE-2, VOS-1, and a putative methyltransferase LAE-1 show light-independent nucleocytoplasmic localization. Two distinct velvet complexes, a heterotrimeric VE-1/VE-2/LAE-1 and a heterodimeric VE-2/VOS-1 are found in vivo. The heterotrimer-complex, which positively regulates sexual development and represses asexual sporulation, suppresses siderophore coprogen production under iron starvation conditions. The VE-1/VE-2 heterodimer controls carotene production. VE-1 regulates the expression of >15% of the whole genome, comprising mainly regulatory and developmental features. We also studied intergenera functions of the velvet complex through complementation of Aspergillus nidulans veA, velB, laeA, vosA mutants with their N. crassa orthologs ve-1, ve-2, lae-1, and vos-1, respectively. Expression of VE-1 and VE-2 in A. nidulans successfully substitutes the developmental and SM functions of VeA and VelB by forming two functional chimeric velvet complexes in vivo, VelB/VE-1/LaeA and VE-2/VeA/LaeA, respectively. Reciprocally, expression of veA restores the phenotypes of the N. crassa ve-1 mutant. All N. crassa velvet proteins heterologously expressed in A. nidulans are localized to the nuclear fraction independent of light. These data highlight the conservation of the complex formation in N. crassa and A. nidulans. However, they also underline the intergenera similarities and differences of velvet roles according to different life styles, niches and ontogenetic processes.