Charge-Dependent Strategy Enables a Single Fluorescent Probe to Study the Interaction Relationship between Mitochondria and Lipid Droplets
Charge-Dependent Strategy Enables a Single Fluorescent Probe to Study the Interaction Relationship between Mitochondria and Lipid Droplets
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电荷依赖性策略使单个荧光探针能够研究线粒体和脂滴之间的相互作用关系
DOI：
10.1021/acssensors.0c02677
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发表时间：
2021-03-23
期刊：
影响因子：
8.9
通讯作者：
Lin, Weiying
中科院分区：
文献类型：
--
作者：
Li, Xuechen;Long, Chenyuan;Lin, Weiying
细胞器之间的合作对维持细胞的正常运转至关重要。脂滴（LD）是一种动态细胞器，专门用于脂质储存，并可与多种细胞类型中的线粒体发生物理相互作用。然而，仍然缺乏原位研究线粒体与LD相互作用关系的合适方法。本文首次提出了一种电荷依赖的策略，充分考虑了线粒体和LD之间的电荷差异。根据这一新策略，我们开发了一种基于环化和开环转化的独特荧光探针Mito-LD。Mito-LD可同时染色线粒体和LD，并分别发出红色和绿色荧光。更重要的是，以Mito-LD为探针，从LD积累、线粒体功能障碍、低温环境和细胞凋亡四个方面详细研究了线粒体LDs的原位相互作用关系。实验结果表明，线粒体在LD的积累中起着重要作用，当线粒体功能障碍时，LD的数量和大小会增加，这可能是由于过量的脂质体所致。此外，作为一种能量储存细胞器，LD在帮助协调线粒体能量供应以应对寒冷方面发挥了重要作用。此外，Mito-LD显示线粒体的极性高于LD。总之，Mito-LD探针可以作为一个潜在的工具，为进一步探索的Dopa-LD相互作用机制，重要的是，电荷依赖的策略是有价值的，设计强大的新的探针，在成像多个细胞器。
Cooperation between organelles is essential to maintain the normal operation of the cell. A lipid droplet (LD), a dynamic organelle, is specialized in lipid storage and can interact physically with mitochondria in several cell types. However, an appropriate method for in situ studying the interaction relationships of mitochondria-LDs is still lacking. Herein, a charge-dependent strategy is proposed for the first time by considering adequately the charge difference between mitochondria and LDs. According to the novel strategy, we have developed a unique fluorescent probe Mito-LD based on the cyclization and ring-opening conversion. Mito-LD could simultaneously stain mitochondria and LDs and emit a red and green fluorescence, respectively. More importantly, with the probe Mito-LD, the in situ interaction relationships of mitochondria-LDs were investigated in detail from LD accumulation, mitochondrial dysfunction, lower environmental temperatures, and four aspects of apoptosis. The experimental results showed that mitochondria played an important role in LD accumulation, and the numbers and size of LDs would increase after mitochondrial dysfunction that may be due to excess liposomes. In addition, as an energy storage organelle, LDs played an important role in helping to coordinate mitochondrial energy supply in response to cold. In addition, the Mito-LD revealed that the polarity of mitochondria was higher than that of LDs. In a word, the probe Mito-LD could serve as a potential tool for further exploring mitochondria-LD interaction mechanisms, and importantly, the charge-dependent strategy is valuable for designing robust new probes in imaging multiple organelles.