Thymosin alpha-l-transformed Bifidobacterium promotes T cell proliferation and maturation in mice by oral administration
Thymosin alpha-l-transformed Bifidobacterium promotes T cell proliferation and maturation in mice by oral administration
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DOI：
10.1016/j.intimp.2012.12.031
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发表时间：
2013-03-01
影响因子：
5.6
通讯作者：
Zeng, Weisen
中科院分区：
文献类型：
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作者：
Shao, Congwen;Tian, Guojun;Zeng, Weisen
胸腺素α-1（T α 1）已被用作免疫增强剂，用于通过注射给药治疗免疫缺陷疾病。但是，注射不方便，可能会引起许多副作用。为了提高T α 1的给药方便性，制备了人T α 1基因转化的长双歧杆菌（BL-T α 1），并研究了其经口给药对小鼠免疫功能的影响。每隔一天用BL-T α 1处理Balb/c小鼠，BL-T α 1用0.2% L-阿拉伯糖预诱导，持续2周。B。以空载体转化的长柄侧耳（BL-0）为阴性对照，生理盐水（NS，0.9%盐水）为空白对照。结果表明：（1）与阴性对照BL-0组相比，BL-T α 1组小鼠血液、脾脏和胸腺中的CD 3（+）CD 4（+）、CD 3（+）CD 8（+）T细胞以及胸腺和脾脏中的CD 4（+）CD 8（+）T细胞均显著升高;（2）BL-T α 1组血清IFN-γ、IL-12水平明显升高。BL-T alpha 1组与BL-0组的肿瘤坏死因子-α（TNF-alpha）和白细胞介素-4（IL-4）水平无明显差异;（3）治疗三个月后，BL-T alpha 1组出现胸腺增生和淋巴结肿大。结论：T alpha 1转化的B.通过口服给药，longum促进胸腺和淋巴结生长，刺激T细胞增殖和成熟，并通过Th 1途径增强细胞免疫。皇冠版权所有（C）2013由爱思唯尔B. V.出版保留所有权利。
Thymosin alpha-1 (T alpha 1) has been used as an immune potentiator for treatment of immune deficiency diseases by injection administration. However, injection is inconvenient and may cause many side effects. In order to improve the administration convenience of T alpha 1, a human T alpha 1 gene transformed Bifidobacterium longum (BL-T alpha 1) was prepared and its effects on mice immunity by oral administration were investigated. The Balb/c mice were treated with BL-T alpha 1, which was pre-induced with 0.2% L-arabinose, every other day for 2 weeks. The B. longum transformed with empty vector (BL-0) was used as the negative control, and normal saline (NS, 0.9% saline) was used as the blank control. The results shown that (1) the CD3(+)CD4(+) and CD3(+)CD8(+) T-cells in blood, spleen and thymus, and the CD4(+)CD8(+) cells in thymus and spleen of BL-T alpha 1 group were all significantly increased than that of negative control BL-0 group respectively; (2) the interferon-gamma (IFN-gamma) and interleukin-12 (IL-12) in serum of BL-T alpha 1 group were significantly increased. No significant differences were found in the levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-4 (IL-4) between BL-T alpha 1 group and BL-0 group; (3) thymic hyperplasia and lymphadenectasis were observed in BL-T alpha 1 group after three-month treatment In conclusion, the T alpha 1-transformed B. longum promotes thymus and lymph nodes growth, stimulates T cell proliferation and maturation, and enhances cellular immunity through Th1 pathway by oral administration. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.