The influence of plasma bilirubin on zinc protoporphyrin measurement by a hematofluorimeter.
The influence of plasma bilirubin on zinc protoporphyrin measurement by a hematofluorimeter.
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血浆胆红素对血荧光计测量锌原卟啉的影响。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
Lubin Bh
中科院分区：
文献类型：
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作者：
E. Buhrmann;Mentzer Wc;Lubin Bh
最近研制了一种荧光计，通过直接测量全血中红细胞ZPP来检测铅中毒和铁缺乏。已发现血浆胆红素荧光影响该技术。在全血样本中，通过人为增加血浆胆红素浓度（在正常范围内和高于正常范围），可升高荧光计测定的ZPP水平。当通过用天然血浆稀释降低血红蛋白浓度时，也观察到ZPP水平升高。当分析高胆红素血症患者的血液样本时，通过该荧光计直接测量ZPP产生的水平显著高于替代提取方法。全血样品中胆红素的光降解并没有减少荧光干扰。虽然胆红素可测量地减少，荧光增加后5小时暴露于光。
A fluorimeter has recently been developed to detect lead poisoning and iron deficiency by measuring erythrocyte ZPP directly from whole blood. Plasma bilirubin fluorescence has been found to influence this technique. ZPP levels determined by the fluorimeter were elevated by artificially increasing the plasma bilirubin concentration, both within and above the normal range, in whole blood samples. A rise in ZPP levels were also observed when the hemoglobin concentration was lowered by dilution with native plasma. When blood samples of patients with hyperbilirubinemia were analyzed, direct measurement of ZPP by this fluorimeter yielded significantly higher levels than did an alternate extraction method. Photodegradation of bilirubin in whole blood samples did not decrease fluorescent interference. Although the bilirubin measurably decreased, fluorescence increased following a 5 hr exposure to light.