RHEB expression in fibroadenomas of the breast
RHEB expression in fibroadenomas of the breast
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DOI：
10.1111/j.1440-1827.2008.02215.x
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发表时间：
2008-04-01
影响因子：
2.2
通讯作者：
Park, Kwang Hwa
中科院分区：
文献类型：
--
作者：
Eom, Minseob;Han, Airi;Park, Kwang Hwa
虽然纤维腺瘤是乳腺良性肿瘤中最常见的一种，但这种肿瘤的特异性基因尚未被确定。微阵列技术用于鉴别纤维腺瘤和浸润性导管癌之间的差异表达基因。利用逆转录-聚合酶链式反应（RT-PCR）进一步探讨了其中一个已鉴定的基因——富集于大脑的RAS同源基因（RHEB）的比较表达。对5例纤维腺瘤患者的组织样本进行了微阵列分析。在纤维腺瘤样本中，HDAC1、ROS1、TNFRSF10A、WASP2、TYRP1、WEE1和RHEB基因的表达水平比正常组织高两倍以上。RHEB的RT-PCR检测显示，与乳腺癌相比，纤维腺瘤中RHEB的表达增加。real-time PCR结果显示，纤维腺瘤组织中RHEB/ β -actin比值平均为1.99，是乳腺癌组织中RHEB/ β -actin比值0.81的2.46倍（P < 0.01）。显微解剖后的免疫组织化学和PCR显示，与纤维腺瘤基质细胞相比，上皮细胞中RHEB的表达增加。因此，RHEB可以作为细胞病理学区分纤维腺瘤和恶性乳腺癌的辅助诊断工具。
Although fibroadenoma is one of the most common types of benign breast tumor, genes specific to the tumor have not been identified. Microarrays were used to identify differentially expressed genes between fibroadenoma and infiltrating ductal carcinoma. The comparative expression of one of the identified genes, RAS homolog enriched in the brain (RHEB), was further explored using reverse transcriptase-polymerase chain reaction (RT-PCR). Microarray analysis was performed on tissue samples from five patients with fibroadenoma. In the fibroadenoma samples, the genes HDAC1, ROS1, TNFRSF10A, WASP2, TYRP1, WEE1, and RHEB were expressed at levels more than twofold higher than in the normal tissues. RT-PCR for RHEB indicated increased expression of RHEB in fibroadenoma compared to breast cancer. When studied with real-time PCR, the average RHEB/beta-actin ratio in fibroadenoma samples was 1.99, 2.46-fold greater than the average RHEB/beta-actin ratio in breast carcinoma of 0.81 (P < 0.01). Immunohistochemistry and PCR followed by microdissection shows increased expression of RHEB in epithelial cells compared to the stromal cells of fibroadenoma. Therefore, RHEB could be used cytopathologically to distinguish fibroadenoma from malignant breast carcinomas as a secondary diagnostic tool.