Antibody-dependent alternate pathway of complement activation in opsonophagocytosis of Porphyromonas gingivalis.
Antibody-dependent alternate pathway of complement activation in opsonophagocytosis of Porphyromonas gingivalis.
复制标题
牙龈卟啉单胞菌调理吞噬作用中补体激活的抗体依赖性替代途径。
DOI：
10.1128/iai.59.6.2105-2109.1991
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发表时间：
1991
影响因子：
3.1
通讯作者：
Arnold,RR
中科院分区：
文献类型：
--
作者：
Cutler,CW;Kalmar,JR;Arnold,RR
已经表明牙龈卟啉单胞菌蛋白水解补体的能力以及其胶囊的产生有助于抵抗多形性白细胞的吞噬作用。在这份报告中，调理作用的血清补体及其活化途径进行了研究，使用个别血清热处理或耗尽的B，C2和C1 q和二价阳离子Mg 2+和Ca 2+。荧光微量测定法用于定量人多形性白细胞对牙龈卟啉单胞菌A7436的吞噬作用。热处理兔抗牙龈卟啉单胞菌血清（RaPg）（56 ℃，30分钟）导致吞噬作用从100%降低到55% +/-5%，而热处理慢性成人牙周病血清消除吞噬作用。热不稳定活性的RaPg完全恢复与MgEGTA螯合的兔血清，但没有EDTA或EGTA螯合的兔血清。添加去除因子B但不去除C2或C1 q的血清恢复了大部分热不稳定活性;然而，由于酵母聚糖的低水平调理作用（可通过EDTA而非MgEGTA进行调理），去除因子B的血清是可疑的。将80微克/毫升的C1 q添加到C1 q耗尽的血清中，恢复了由于热处理或C1 q耗尽而失去的活性，但不是全部。加入4 × 10（-3）M Mg 2+可增强C2和C1 q缺失血清的大部分调理素活性。这些数据表明，虽然牙龈卟啉单胞菌A7436的调理吞噬作用依赖于经典的补体途径，但抗体依赖性替代途径做出了显著贡献。
It has been suggested that the ability of Porphyromonas gingivalis to proteolyse complement, as well as its production of a capsule, contributes to resistance to phagocytosis by polymorphonuclear leukocytes. In this report, the opsonic role of serum complement and its activation pathways were investigated, using individual sera heat treated or depleted of factors B, C2, and C1q and the divalent cations Mg2+ and Ca2+. A fluorochrome microassay was used to quantitate phagocytosis of P. gingivalis A7436 by human polymorphonuclear leukocytes. Heat treatment of rabbit antiserum to P. gingivalis (RaPg) (56 degrees C, 30 min) resulted in a reduction in phagocytosis from 100% to 55% +/- 5%, while heat treatment of chronic adult periodontal disease serum abrogated phagocytosis. The heat-labile activity of RaPg was fully restored with MgEGTA-chelated rabbit serum but not EDTA- or EGTA-chelated rabbit serum. The addition of serum depleted of factor B but not C2 or C1q restored most of the heat-labile activity; however, the factor B-depleted serum was suspect, due to low-level opsonization of zymosan (inhibitable by EDTA but not MgEGTA). Adding C1q at 80 micrograms/ml to serum depleted of C1q restored much but not all of the activity lost through heat treatment or through depletion of C1q. A large part of opsonic activity with C2- and C1q-depleted sera was enhanced by the addition of 4 x 10(-3) M Mg2+. The data indicate that although opsonophagocytosis of P. gingivalis A7436 is dependent on the classical complement pathway, a significant contribution is made by an antibody-dependent alternate pathway.