Dietary 1,3-diacylglycerol protects against diet-induced obesity and insulin resistance
Dietary 1,3-diacylglycerol protects against diet-induced obesity and insulin resistance
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DOI：
10.1016/j.metabol.2007.06.024
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发表时间：
2007-11-01
影响因子：
9.8
通讯作者：
Ginsberg, Henry N.
中科院分区：
文献类型：
--
作者：
Saito, Shinichiro;Hemandez-Ono, Antonio;Ginsberg, Henry N.
为了研究膳食1，3-二酰基甘油（DAG）对胰岛素抵抗（IR）和肥胖症发展的影响，从8周龄开始，向棕色脂肪组织缺陷小鼠（高脂肪膳食诱导的IR和肥胖症模型）喂食含有DAG油（n = 8）或标准三酰基甘油（TAG）油（n = 9）的西式饮食（WTD）15周。尽管棕色脂肪组织缺陷小鼠在TAG和DAG富集的WTD（TAG-WTD和DAG-WTD）上变得肥胖，但食用DAG-WTD的小鼠体重增加较少，并且体脂积累较少。每种WTD给药5周后进行的葡萄糖耐量试验结果无差异。然而，在每次WTD 10周后，TAG-WTD组中出现了葡萄糖耐量受损，但DAG-WTD可以预防。基因表达的探索性分析表明，DAG-WTD的消耗与肝脏中磷酸烯醇式丙酮酸羧激酶基因表达减少和骨骼肌中过氧化物酶体增殖物激活受体a、脂蛋白脂肪酶和解偶联蛋白2和3基因表达增加相关。DAG-WTD对空腹和餐后血浆甘油三酯（TG）水平、肝脏TG含量或肝脏TG分泌速率无影响。这些发现表明，富含1，3-DAG油的饮食可以通过抑制肝脏中的脂肪生成和刺激骨骼肌中的脂肪氧化来减少WTD诱导的IR和体脂积累。(C)2007年爱思唯尔公司All rights reserved.
To investigate the effect of dietary 1,3-diacylglycerol (DAG) on the development of insulin resistance (IR) and obesity, brown adipose tissue-deficient mice, a model of high-fat diet-induced IR and obesity, were fed Western-type diets (WTD) containing either DAG oil (n = 8) or standard triacylglycerol (TAG) oil (n = 9) for 15 weeks, beginning at 8 weeks of age. Although brown adipose tissue-deficient mice became obese on both TAG- and DAG-enriched WTD (TAG-WTD and DAG-WTD), the mice eating DAG-WTD gained less weight and had less body fat accumulation. The results of glucose tolerance tests conducted after 5 weeks of each WTD were not different. However, after 10 weeks of each WTD, impaired glucose tolerance developed in the TAG-WTD group but was prevented by DAG-WTD. Exploratory analyses of gene expression suggested that consumption of DAG-WTD was associated with reduced phosphoenolpyruvate carboxykinase gene expression in liver and increased expression of the genes for peroxisome proliferator-activated receptor a, lipoprotein lipase, and uncoupling proteins 2 and 3 in skeletal muscle. There were no effects of the DAG-WTD on fasting and postprandial plasma triglyceride (TG) levels, hepatic TG content, or the rate of secretion of TG from the liver. These findings suggest that diets enriched in 1,3-DAG oil may reduce WTD-induced IR and body fat accumulation by suppressing gluconeogenesis in liver and stimulating fat oxidation in skeletal muscle. (C) 2007 Elsevier Inc. All rights reserved.