Effects of sprint training combined with vegetarian or mixed diet on muscle carnosine content and buffering capacity
Effects of sprint training combined with vegetarian or mixed diet on muscle carnosine content and buffering capacity
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DOI：
10.1007/s00421-011-1877-4
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发表时间：
2011-10-01
影响因子：
3
通讯作者：
Derave, Wim
中科院分区：
文献类型：
--
作者：
Baguet, Audrey;Everaert, Inge;Derave, Wim
肌肽是人体骨骼肌中含量丰富的一种具有质子缓冲能力的二肽。关于训练是否可以增加肌肉肌肽从而提供增加缓冲能力的机制存在争议。本研究探讨了5周的短跑训练结合素食或混合饮食对肌肉肌肽、肌肽合成酶mRNA表达和肌肉缓冲能力的影响。20名杂食受试者参加了为期5周的短跑训练干预（每周2-3次）。他们被随机分为素食和混合饮食组。测量（干预期之前和之后）包括通过质子磁共振波谱（（1）H-MRS）测量比目鱼肌、腓肠肌外侧肌和胫骨前肌中的肌肽含量，腓肠肌外侧肌的真实切割活检以确定体外非碳酸氢盐肌肉缓冲能力、肌肽含量（HPLC方法）和肌肽合成酶（CARNS）mRNA表达以及6 x 6 s重复冲刺能力（RSA）测试。比目鱼肌肽含量存在显著的饮食X训练相互作用，混合饮食无显著增加（+11%），素食无显著降低（-9%）。其他肌肉肌肽含量和腓肠肌缓冲能力不受训练影响。CARNS mRNA表达与训练无关，但在素食组中显着下降。RSA测试期间的表现通过训练得到改善，组间无差异。我们发现，肌肉肌肽定量的侵入性和非侵入性方法之间呈正相关（r = 0.517; p = 0.002）。本研究表明，5周短跑训练对肌肉肌肽含量和肌肽合成酶mRNA没有影响。
Carnosine is an abundant dipeptide in human skeletal muscle with proton buffering capacity. There is controversy as to whether training can increase muscle carnosine and thereby provide a mechanism for increased buffering capacity. This study investigated the effects of 5 weeks sprint training combined with a vegetarian or mixed diet on muscle carnosine, carnosine synthase mRNA expression and muscle buffering capacity. Twenty omnivorous subjects participated in a 5 week sprint training intervention (2-3 times per week). They were randomized into a vegetarian and mixed diet group. Measurements (before and after the intervention period) included carnosine content in soleus, gastrocnemius lateralis and tibialis anterior by proton magnetic resonance spectroscopy ((1)H-MRS), true-cut biopsy of the gastrocnemius lateralis to determine in vitro non-bicarbonate muscle buffering capacity, carnosine content (HPLC method) and carnosine synthase (CARNS) mRNA expression and 6 x 6 s repeated sprint ability (RSA) test. There was a significant diet x training interaction in soleus carnosine content, which was non-significantly increased (+11%) with mixed diet and non-significantly decreased (-9%) with vegetarian diet. Carnosine content in other muscles and gastrocnemius buffer capacity were not influenced by training. CARNS mRNA expression was independent of training, but decreased significantly in the vegetarian group. The performance during the RSA test improved by training, without difference between groups. We found a positive correlation (r = 0.517; p = 0.002) between an invasive and non-invasive method for muscle carnosine quantification. In conclusion, this study shows that 5 weeks sprint training has no effect on the muscle carnosine content and carnosine synthase mRNA.