THE INDUCTION OF LONG-TERM POTENTIATION IN SPINAL DORSAL HORN AFTER PERIPHERAL NOCICEPTIVE STIMULATION AND CONTRIBUTION OF SPINAL TRPV1 IN RATS
THE INDUCTION OF LONG-TERM POTENTIATION IN SPINAL DORSAL HORN AFTER PERIPHERAL NOCICEPTIVE STIMULATION AND CONTRIBUTION OF SPINAL TRPV1 IN RATS
复制标题
大鼠外周伤害性刺激后脊髓背角长时程增强的诱导及脊髓TRPV1的贡献
DOI：
10.1016/j.neuroscience.2014.03.037
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发表时间：
2014-06-06
期刊：
影响因子：
3.3
通讯作者：
Wan, Y.
中科院分区：
文献类型：
--
作者：
Yang, F.;Guo, J.;Wan, Y.
在慢性疼痛状态下，外周伤害性刺激可诱导脊髓背角的长时程增强 (LTP)，但尚不清楚外周伤害性刺激后脊髓 LTP 的发展速度有多快。此外，瞬时受体电位香草酸1型（TRPV1）受体在脊髓背角中丰富，特别是在浅层，并且被认为与突触可塑性有关。在这项研究中，我们研究了炎症损伤和电刺激后 LTP 诱导的时间范围以及 TRPV1 受体的参与。通过细胞外浅表脊髓背角C纤维诱发场电位记录和体内梳理纤维记录，我们发现皮下注射完全弗氏佐剂（CFA）或5%福尔马林可在3 h内诱导脊髓背角C纤维诱发场电位的低频、不规则放电和LTP。在脊髓上局部应用 TRPV1 受体拮抗剂辣椒西平可抑制 CFA 或福尔马林对脊髓 LTP 的诱导。此外，capsazepine和另一种TRPV1拮抗剂(E)-3-(4-t-丁基苯基)-N-(2,3-二氢苯并[b][1,4]二恶英-6-基)丙烯酰胺部分或完全阻断了高频和低频电刺激条件刺激诱导的LTP。这些结果表明，CFA 或 5% 福尔马林的急性外周炎症刺激可以在刺激发生后很早就诱导脊髓 LTP，并且脊髓背角中的 TRPV1 受体可能有助于这种 LTP 诱导。 (C) 2014 年国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
During chronic pain states, peripheral nociceptive stimulation can induce long-term potentiation (LTP) in the spinal dorsal horn, but it is not clear how quickly spinal LTP develops after peripheral noxious stimulation. Furthermore, transient receptor potential vanilloid type 1 (TRPV1) receptors are abundant in spinal cord dorsal horn, especially in the superficial layers, and are thought to be involved in synaptic plasticity. In this study, we investigated the time frame of LTP induction after inflammatory insult and electrical stimulation and the involvement of TRPV1 receptors. By using extracellular recordings of C-fiber-evoked field potentials in the superficial spinal dorsal horn and teased fiber recording in vivo, we found that subcutaneous injection of complete Freund's adjuvant (CFA) or 5% formalin induced low-frequency, irregular discharges of C-fibers and LTP of the C-fiber-evoked field potentials in the spinal dorsal horn within 3 h. Topical application of the TRPV1 receptor antagonist capsazepine onto the spinal cord inhibited the induction of spinal LTP by CFA or formalin. Furthermore, capsazepine and another TRPV1 antagonist, (E)-3-(4-t-butylphenyl)-N-(2,3-dihydrobenzo[b][1,4]dioxin-6-yl)acrylamide, partially or completely blocked the LTP induced by conditioning stimulation with high-and low-frequency electrical stimulation. These results suggest that acute peripheral inflammatory stimulation by CFA or 5% formalin can induce spinal LTP very early after stimulation onset and that TRPV1 receptors in the spinal dorsal horn might contribute to this LTP induction. (C) 2014 IBRO. Published by Elsevier Ltd. All rights reserved.