A reassortant Bunyavirus isolated from acute hemorrhagic fever cases in Kenya and Somalia
A reassortant Bunyavirus isolated from acute hemorrhagic fever cases in Kenya and Somalia
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DOI：
10.1006/viro.2001.1201
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发表时间：
2001-12-20
期刊：
影响因子：
3.7
通讯作者：
Nichol, ST
中科院分区：
文献类型：
--
作者：
Bowen, MD;Trappier, SG;Nichol, ST
1997年底和1998年初，东非爆发大规模出血热疫情。在肯尼亚和索马里南部收集了临床样本，通过 IgM 检测、病毒分离、逆转录聚合链反应 (RT-PCR) 检测病毒 RNA 或免疫组织化学确定，115 名出血热患者中的 27 名 (23%) 显示出急性裂谷热 (RVF) 病毒感染的证据。然而，两名符合出血热病例定义的患者（一名来自肯尼亚，另一名来自索马里）产生的病毒分离株并非裂谷热病毒。电子显微镜表明这两种病毒分离株是布尼亚病毒科的成员。 RT-PCR 引物旨在检测这些样本中的布尼亚病毒 RNA。两个分离株的 S 和 L 片段区域被成功扩增，它们的核苷酸序列与 Bunyamwera 病毒几乎完全一致，这是一种蚊媒病毒，以前与严重的人类疾病无关。出乎意料的是，病毒 M 片段似乎是重配的，因为检测到的序列与 Bunyamwera 病毒的相应 M 片段序列相比，显示出 32-33% 的核苷酸和 28% 的氨基酸差异。在该地区另外 12 例出血热病例的急性期血清中检测到的病毒 RNA，支持了这种重配的布尼亚病毒（拟定名为加里萨病毒）与严重疾病的关联。
In late 1997 and early 1998, a large outbreak of hemorrhagic fever occurred in East Africa. Clinical samples were collected in Kenya and southern Somalia, and 27 of 115 (23%) hemorrhagic fever patients tested showed evidence of acute infection with Rift Valley fever (RVF) virus as determined by IgM detection, virus isolation, detection of virus RNA by reverse transcription-polymerise chain reaction (RT-PCR), or immunohistochemistry. However, two patients (one from Kenya and the other from Somalia) whose illness met the hemorrhagic fever case definition yielded virus isolates that were not RVF Electron microscopy suggested these two virus isolates were members of the family Bunyaviridae. RT-PCR primers were designed to detect bunyavirus RNA in these samples. Regions of the S and L segments of the two isolates were successfully amplified, and their nucleotide sequences exhibited nearly complete identity with Bunyamwera virus, a mosquito-borne virus not previously associated with severe human disease. Unexpectedly, the virus M segment appeared to be reassorted, as the sequences detected exhibited 32-33% nucleotide and 28% amino acid differences relative to the corresponding M segment sequence of Bunyamwera virus, The association of this reassortant bunyavirus, proposed name Garissa virus, with severe disease is supported by the detection of the virus RNA in acute-phase sera taken from 12 additional hemorrhagic fever cases in the region.