Comparative Mitochondrial Genome Analysis of Eligma narcissus and other Lepidopteran Insects Reveals Conserved Mitochondrial Genome Organization and Phylogenetic Relationships.
Comparative Mitochondrial Genome Analysis of Eligma narcissus and other Lepidopteran Insects Reveals Conserved Mitochondrial Genome Organization and Phylogenetic Relationships.
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DOI：
10.1038/srep26387
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发表时间：
2016-05-25
影响因子：
4.6
通讯作者：
Liu CL
中科院分区：
文献类型：
--
作者：
Dai LS;Zhu BJ;Zhao Y;Zhang CF;Liu CL
本研究测定了水仙麦蛾的线粒体全基因组序列，并与其它18种鳞翅目昆虫进行了比较。线粒体基因组（mitogenome）是一个15，376 bp的环状分子，包含13个蛋白质编码基因（PCG），22个转运RNA（tRNA）基因，两个核糖体RNA（rRNA）基因和一个腺嘌呤（A）+胸腺嘧啶（T）-丰富区域。正的AT偏斜（0.007）表明发生的As多于Ts。其中13个PCG的排列与已测序的鳞翅目昆虫相似。所有PCG启动ATN密码子，除了细胞色素c氧化酶亚基1（cox 1）基因，这是由CGA序列，在其他鳞翅目昆虫中观察到的。密码子使用分析结果表明，Asn、Ile、Leu、Tyr和Phe是5种最常见的氨基酸。所有的tRNA基因都被折叠成预期的典型三叶草结构，观察线粒体tRNA基因。根据其他昆虫有丝分裂基因组中13个PCG的核苷酸序列分析了系统发育关系，证实E.水仙属夜蛾科。
In this study, we sequenced the complete mitochondrial genome of Eligma narcissus and compared it with 18 other lepidopteran species. The mitochondrial genome (mitogenome) was a circular molecule of 15,376 bp containing 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes and an adenine (A) + thymine (T) − rich region. The positive AT skew (0.007) indicated the occurrence of more As than Ts. The arrangement of 13 PCGs was similar to that of other sequenced lepidopterans. All PCGs were initiated by ATN codons, except for the cytochrome c oxidase subunit 1 (cox1) gene, which was initiated by the CGA sequence, as observed in other lepidopterans. The results of the codon usage analysis indicated that Asn, Ile, Leu, Tyr and Phe were the five most frequent amino acids. All tRNA genes were shown to be folded into the expected typical cloverleaf structure observed for mitochondrial tRNA genes. Phylogenetic relationships were analyzed based on the nucleotide sequences of 13 PCGs from other insect mitogenomes, which confirmed that E. narcissus is a member of the Noctuidae superfamily.