Actinomadura deserti sp nov., isolated from desert soil
Actinomadura deserti sp nov., isolated from desert soil
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Actinomadura Deserti sp nov.，从沙漠土壤中分离出来
DOI：
10.1099/ijsem.0.002922
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发表时间：
2018
影响因子：
2.8
通讯作者：
Huang Ying
中科院分区：
文献类型：
--
作者：
Cao Chengliang;Xu Tangyu;Liu Jinjuan;Cai Xiaorui;Sun Yong;Qin Sheng;Jiang Jihong;Huang Ying
从中国西北新疆省收集的沙漠土壤中分离出一株革兰氏阳性、严格需氧的放线菌，命名为 BMP B8004T。它产生了广泛分枝的、不断裂的基质菌丝体和非常稀少的气生菌丝体，形成了具有光滑表面的短钩状节孢子链。最佳生长条件为 28°C、pH 7.0 和 0% (w/v) NaCl。基于 16S rRNA 基因序列的系统发育分析表明，BMP B8004T 菌株在马杜拉放线菌属内形成了独特的系统谱系。它与Actinomadura apisIM17-1T (99.2 %)和Actinomadura rifamyciniNBRC 14183T(98.6 %)具有最高的16S rRNA基因序列相似性。然而，根据低水平的 DNA-DNA 相关性（分别为 52.7±0.7% 和 45±1.8%），它可以与两个最接近的菌株区分开来。化学分类学特征，包括主要磷脂、二磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇和磷脂酰肌醇甘露糖苷、主要甲基萘醌MK-9(H6)和MK-9(H8)、主要脂肪酸iso-C16 : 0、C16 : 0、 C18 : 010-甲基和C18 : 1ω9c也与Actinomadura属的特性一致。菌株BMP B8004T的DNA G+C 含量为71.9 mol％。基于表型和基因型特征，菌株BMP B8004Ti被认为代表马杜拉放线菌属的新种，其名称为Actinomadura Desertisp。十一月被提议。模式菌株是BMP B8004T(=CGMCC 4.7432T=KCTC 39998T)。
A Gram-positive, strictly aerobic actinobacterium, designated BMP B8004T, was isolated from desert soil collected in Xinjiang Province, Northwest China. It produced an extensively branched non-fragmenting substrate mycelium, and very scanty aerial mycelium that formed a short hooked chain of arthrospores with a smooth surface. Optimum growth occurred at 28 °C, pH 7.0 and 0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BMP B8004Tformed a distinct phyletic lineage within the genusActinomadura. It shared the highest 16S rRNA gene sequence similarity toActinomadura apisIM17-1T(99.2 %) andActinomadura rifamyciniNBRC 14183T(98.6 %). However, it could be distinguished from the two closest strains based on the low levels of DNA–DNA relatedness (52.7±0.7 and 45±1.8 %, respectively). Chemotaxonomic characteristics, including the main phospholipids, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides, the major menaquinones MK-9(H6) and MK-9(H8), the predominant fatty acids iso-C16 : 0, C16 : 0, C18 : 010-methyl and C18 : 1ω9c, were also consistent with the properties of the genusActinomadura. The DNA G+C content of strain BMP B8004Twas 71.9 mol%. Based on phenotypic and genotypic features, strain BMP B8004Tis considered to represent a novel species of the genusActinomadura, for which the nameActinomadura desertisp. nov. is proposed. The type strain is BMP B8004T(=CGMCC 4.7432T=KCTC 39998T).