Molecular evolution of the SARS coronavirus during the course of the SARS epidemic in China
Molecular evolution of the SARS coronavirus during the course of the SARS epidemic in China
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DOI：
10.1126/science.1092002
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发表时间：
2004-03-12
期刊：
影响因子：
56.9
通讯作者：
Lo, YMD
中科院分区：
文献类型：
--
作者：
He, JF;Peng, GW;Lo, YMD
61 SARS冠状病毒的基因组序列来自严重急性呼吸综合征（SARS）流行的早期，中期和晚期阶段一起分析了两个病毒序列从果子狸。发现了各阶段的基因型特征，最早的基因型与动物SARS样冠状病毒相似。在流行病开始和结束时，在基因组的Orf 8区域观察到主要缺失。病毒基因组的中性突变率是恒定的，但编码序列的氨基酸取代率在流行过程中减慢。穗蛋白表现出最强的初始反应，积极的选择压力，随后纯化选择和最终稳定。
Sixty-one SARS coronavirus genomic sequences derived from the early, middle, and late phases of the severe acute respiratory syndrome ( SARS) epidemic were analyzed together with two viral sequences from palm civets. Genotypes characteristic of each phase were discovered, and the earliest genotypes were similar to the animal SARS-like coronaviruses. Major deletions were observed in the Orf8 region of the genome, both at the start and the end of the epidemic. The neutral mutation rate of the viral genome was constant but the amino acid substitution rate of the coding sequences slowed during the course of the epidemic. The spike protein showed the strongest initial responses to positive selection pressures, followed by subsequent purifying selection and eventual stabilization.