Distinct Subtypes of Cholecystokinin (CCK)-Containing Interneurons of the Basolateral Amygdala Identified Using a CCK Promoter-Specific Lentivirus
Distinct Subtypes of Cholecystokinin (CCK)-Containing Interneurons of the Basolateral Amygdala Identified Using a CCK Promoter-Specific Lentivirus
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DOI：
10.1152/jn.91149.2008
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发表时间：
2009-03-01
影响因子：
2.5
通讯作者：
Rainnie, Donald G.
中科院分区：
文献类型：
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作者：
Jasnow, Aaron M.;Ressler, Kerry J.;Rainnie, Donald G.
Jasnow AM，Ressler KJ，Hammack SE，Chhatwal JP，Rainnie DG.使用CCK启动子特异性慢病毒鉴定基底外侧杏仁核含胆囊收缩素中间神经元的不同亚型。神经生理学杂志101：1494-1506，2009年。首次发表于2009年1月21日; doi：10.1152/jn.91149.2008。基底外侧杏仁核（BLA）对情感记忆的形成至关重要。BLA中间神经元的生理特性知之甚少，根据其免疫细胞化学特征可分为四种亚型。胆囊收缩素（CCK）中间神经元在前馈抑制和行为恐惧反应中起重要作用。有证据表明，一个亚组内的中间神经元可以显示异质性的生理特性。然而，很少有人知道的生理特性的CCK中间神经元在BLA和/或他们是否代表一个同质或异质的人口。为了解决这个问题，我们产生了一个慢病毒表达GFP的CCK启动子的控制下，以确定CCK神经元在体内。我们结合全细胞膜片钳记录技术来研究大鼠BLA含CCK的中间神经元的生理特性。在这里，我们描述了57个细胞记录在电流钳模式的生理特性，我们使用分层聚类和判别函数分析，证明CCK中间神经元可以分为三个不同的亚型（I，II，III）的基础上，他们的被动和主动膜性能。此外，II型神经元可以进一步分为适应和非适应类型的基础上，他们的尖峰频率适应率。这些数据表明，BLA的CCK中间神经元是一个异质性的人口，可能是功能不同的亚群，差异有助于处理情绪突出的刺激。
Jasnow AM, Ressler KJ, Hammack SE, Chhatwal JP, Rainnie DG. Distinct subtypes of cholecystokinin-containing interneurons of the basolateral amygdala identified using a CCK promoter-specific lentivirus. J Neurophysiol 101: 1494-1506, 2009. First published January 21, 2009; doi:10.1152/jn.91149.2008. The basolateral amygdala (BLA) is critical for the formation of emotional memories. Little is known about the physiological properties of BLA interneurons, which can be divided into four subtypes based on their immunocytochemical profiles. Cholecystokinin (CCK) interneurons play critical roles in feedforward inhibition and behavioral fear responses. Evidence suggests that interneurons within a subgroup can display heterogeneous physiological properties. However, little is known about the physiological properties of CCK interneurons in the BLA and/or whether they represent a homogeneous or heterogeneous population. To address this question, we generated a lentivirus-expressing GFP under the control of the CCK promoter to identify CCK neurons in vivo. We combined this with whole cell patch-clamp recording techniques to examine the physiological properties of CCK-containing interneurons of the rat BLA. Here, we describe the physiological properties of 57 cells recorded in current-clamp mode; we used hierarchical cluster and discriminant function analysis to demonstrate that CCK interneurons can be segregated into three distinct subtypes (I, II, III) based on their passive and active membrane properties. Additionally, Type II neurons could be further separated into adapting and nonadapting types based on their rates of spike frequency adaptation. These data suggest that CCK interneurons of the BLA are a heterogeneous population and may be functionally distinct subpopulations that differentially contribute to the processing of emotionally salient stimuli.