The development and evaluation of a continuous flow process for the lipase-mediated oxidation of alkenes
The development and evaluation of a continuous flow process for the lipase-mediated oxidation of alkenes
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DOI：
10.3762/bjoc.5.27
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发表时间：
2009-06-02
影响因子：
2.7
通讯作者：
Watts, Paul
中科院分区：
文献类型：
--
作者：
Wiles, Charlotte;Hammond, Marcus J.;Watts, Paul
我们报告了使用固定化形式的南极洲假丝酵母脂肪酶B，Novozym(R)435，在开发连续流动反应器中的初步调查，该反应器能够利用商业上可获得的氧化剂过氧化氢(100卷)高产率和高纯度地执行烯烃的化学-酶氧化。最初的研究集中在脂肪酶介导的1-甲基环己烯的氧化，随后优化的反应条件用于一系列芳香族和脂肪族烯烃的环氧化，产率和定量纯度为97.6%至99.5%。
We report the use of an immobilised form of Candida antarctica lipase B, Novozym (R) 435, in a preliminary investigation into the development of a continuous flow reactor capable of performing the chemo-enzymatic oxidation of alkenes in high yield and purity, utilising the commercially available oxidant hydrogen peroxide (100 volumes). Initial investigations focussed on the lipase-mediated oxidation of 1-methylcyclohexene, with the optimised reaction conditions subsequently employed for the epoxidation of an array of aromatic and aliphatic alkenes in 97.6 to 99.5% yield and quantitative purity.