AINTEGUMENTA-LIKE6 regulates cellular differentiation in flowers
AINTEGUMENTA-LIKE6 regulates cellular differentiation in flowers
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DOI：
10.1007/s11103-011-9844-3
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发表时间：
2012-02-01
影响因子：
5.1
通讯作者：
Eaddy, Marcie
中科院分区：
文献类型：
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作者：
Krizek, Beth A.;Eaddy, Marcie
在花发育过程中，花分生组织中的多能干细胞产生增殖前体细胞，其后代最终在每个花器官中获得专门的功能。植物细胞从增殖状态向分化状态转变的调控机制尚不清楚。AINTEGUMENTA- like / glut （AIL/PLT）转录因子家族的几个成员，包括AINTEGUMENTA （ANT）和AIL6/PLT3，是花细胞增殖的重要调节因子。为了进一步研究AIL6在花发育中的作用，我们对AIL6编码区在组成型35S启动子（35S:cAIL6）控制下表达的转基因植物进行了表征。这些植物显示出花器官大小和形态的变化，这些变化与发育器官中细胞分裂的模式和持续时间的改变有关。此外，我们发现非常高水平的AIL6表达抑制细胞分化。相比之下，蚂蚁ail6双突变体表现出花分生组织细胞的过早分化。这些结果表明，这两个转录因子在花的增殖和分化中都起着调节作用。
During flower development, pluripotent stem cells within the floral meristem give rise to proliferative precursor cells whose progeny eventually acquire specialized functions within each floral organ. The regulatory mechanisms by which plant cells transition from a proliferating state to a differentiated state are not well characterized. Several members of the AINTEGUMENTA-LIKE/PLETHORA (AIL/PLT) transcription factor family, including AINTEGUMENTA (ANT) and AIL6/PLT3, are important regulators of cell proliferation in flowers. To further investigate the role of AIL6 during flower development, we have characterized transgenic plants in which the coding region of AIL6 was expressed under the control of the constitutive 35S promoter (35S:cAIL6). These plants display changes in floral organ size and morphology that are associated with alterations in the pattern and duration of cell divisions within developing organs. In addition, we find that very high levels of AIL6 expression inhibit cellular differentiation. In contrast, ant ail6 double mutants display premature differentiation of floral meristem cells. These results indicate that these two transcription factors regulate both proliferation and differentiation in flowers.