Sericin enhances the developmental competence of heat-stressed bovine embryos
Sericin enhances the developmental competence of heat-stressed bovine embryos
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DOI：
10.1002/mrd.23038
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发表时间：
2018-08-01
影响因子：
2.5
通讯作者：
Yamanaka, Ken-ichi
中科院分区：
文献类型：
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作者：
Khatun, Hafiza;Egashira, Junki;Yamanaka, Ken-ichi
我们研究了丝胶对热应激(HS)下牛胚胎发育能力的影响。第2天HS显著降低第8天的囊胚发育率和线粒体活性，显著增加细胞内活性氧和末端脱氧核苷酸转移酶生物素-dUTP缺口末端标记(TUNEL)阳性细胞的数量，而第7天HS仅显著降低第8天囊胚的线粒体活性和TUNEL阳性细胞数。这些有害的影响可以通过补充丝胶中和。接下来，为了从耐热性的角度研究获得高活力囊胚的可能性，将胚胎与丝胶一起培养到第7天，然后在无丝胶的培养液中暴露于HS。丝胶培养的囊胚TUNEL阳性细胞数显著低于对照囊胚。丝胶培养产生的囊胚中，HSPA1A和Bax的转录丰度显着降低，而IFNT2水平显着升高。综上所述，这些发现证明了丝胶的抗氧化和抗凋亡活性，以及丝胶在体外生产高活力胚胎的潜在用途。
We investigated the effects of sericin on the developmental competence of bovine embryos exposed to heat stress (HS). Putative zygotes were cultured with sericin and subjected to HS (40.5 degrees C for 6hr) on Day 2 or 7 followed by continuous culture at 38.5 degrees C until Day 8. Day 2 HS significantly decreased blastocyst development on Day 8 as well as mitochondrial activity, and significantly increased the amount of intracellular reactive oxygen species and terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL)-positive cells, whereas Day 7 HS only significantly decreased mitochondrial activity and increased the number of TUNEL-positive cells in Day 8 blastocysts. These detrimental effects were neutralized by sericin supplementation. Next, to investigate the potential production of blastocysts with high viability in terms of thermotolerance, embryos were cultured with sericin until Day 7, and then exposed to HS in the sericin-free medium. TUNEL-positive cell numbers were significantly lower in blastocysts produced by sericin culture than in control blastocysts. Transcript abundance for HSPA1A and BAX was significantly decreased but IFNT2 levels were increased in blastocysts produced by sericin culture. In conclusion, these findings demonstrate the anti-oxidative and anti-apoptotic activities of sericin, and the potential use of sericin to produce embryos with high viability in vitro.