A novel genetic marker to differentiate feline herpesvirus type 1 field isolates
A novel genetic marker to differentiate feline herpesvirus type 1 field isolates
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DOI：
10.1016/j.vetmic.2004.12.028
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发表时间：
2005-04-10
影响因子：
3.3
通讯作者：
Akashi, H
中科院分区：
文献类型：
--
作者：
Hamano, M;Maeda, K;Akashi, H
用限制性内切酶SalI或MluI消化比较了5个猫疱疹病毒I型（FHV-1）的最新野外分离株。SalI消化显示，在一个分离株00-035中存在潜在有用的差异，该分离株具有约3.0 kbp的片段，而不是其他菌株中的2.6 kbp片段。在克隆3.0和2.6kbp片段后，分析核苷酸序列。结果表明，3.0 kbp的00-035片段包含一个完整的单纯疱疹病毒1型（HSV-1）UL 17基因同源物的开放阅读框架和UL 16基因的5 '端部分，仅在2.6 kbp片段的SalI位点所在的UL 17基因5'端区域发现一个核苷酸替换。基于这些核苷酸序列，设计两个PCR引物以扩增UL 17基因中SalI位点周围的区域，并使用来自日本各地的78个田间分离株进行PCR。PCR产物的SalI酶切结果显示，UL 17基因中不含SalI位点的基因型在鸟取县和山形县占优势（分别为69%和75%），而在日本其他地区占次要地位（0-10%）。这些结果表明，本研究中描述的SalI酶切方法可以用作遗传标记来区分一些FHV-1田间分离物，这是第一个报告，显示不同的分布FHV-1基因型使用新的遗传标记。(c)2005 Elsevier B. V.保留所有权利。
Five recent field isolates of feline herpesvirus type I (FHV-1) were compared by digestion with a restriction endonuclease, SalI or MluI. The SalI digestion showed a potentially useful difference in one isolate 00-035 that had an approximately 3.0 kbp fragment instead of a 2.6 kbp fragment in the other strains. After cloning the 3.0 and 2.6 kbp fragments, the nucleotide sequences were analyzed. The result showed that the 3.0 kbp fragment of 00-035 included a complete open reading frame of the herpes simplex virus 1 (HSV-1) homologue of the UL17 gene and a 5'-part of UL16 gene and that only one nucleotide substitution was found in the 5'-region of UL17 gene where the SalI site of the 2.6 kbp fragment locates. Based on these nucleotide sequences, two PCR primers were designed to amplify the region around the SalI site in the UL17 gene and the PCR was carried out using 78 field isolates from various parts of Japan. The SalI digestion of the PCR products revealed an interesting profile in that the genotype without the SalI site in UL17 gene was dominant in Tottori and Yamagata prefectures (69% and 75%, respectively) but minor in the other regions of Japan (0-10%). These results suggest that the SalI digestion method described in the present study can be used as a genetic marker to differentiate some FHV-1 field isolates and this is the first report that showed different distributions of FHV-1 genotypes using the novel genetic marker. (c) 2005 Elsevier B.V. All rights reserved.