Endothelin: visualization of mRNAs by in situ hybridization provides evidence for local action.
Endothelin: visualization of mRNAs by in situ hybridization provides evidence for local action.
复制标题
内皮素：通过原位杂交观察 mRNA 的情况，为局部作用提供了证据。
DOI：
10.1073/pnas.86.18.7285
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发表时间：
1989
影响因子：
11.1
通讯作者：
Snyder,SH
中科院分区：
文献类型：
--
作者：
MacCumber,MW;Ross,CA;Glaser,BM;Snyder,SH
内皮素（ET）是一种新近发现的血管活性肽，有三种亚型，已克隆了三个基因。该肽的合成的细胞位点尚未在体内鉴定。利用北方印迹分析，我们已经检测到两种形式的ET mRNA在大鼠组织中：3.7-脱氢酶的形式在肾脏，眼睛，和脑，2.5-脱氢酶的形式在肠，和两种形式在肺。我们已经本地化这些形式的ET mRNA在几个大鼠组织中使用原位杂交。在19天的胎鼠中，ET mRNA在肺、肠和脑膜中最高。在高分辨率下，ET mRNA定位于肺细支气管的呼吸上皮细胞和血管中。在成人组织中，ET mRNA存在于整个肺、肾髓质直血管和眼睛的虹膜中。ET mRNA在许多器官中的ET结合位点附近合成（例如，肺、肾、肠和眼），表明该肽的局部作用。然而，在其他领域（例如，心脏和肾皮质），ET结合位点在缺乏ET mRNA的情况下存在，表明ET的作用来自血流或神经元。培养的微血管内皮细胞中ET mRNA的北方印迹分析表明，ET在小血管中合成，并且与其在大血管中的调节类似。我们的研究结果提供的证据表明，ET，像其他调节肽，可能在几个组织中作为一种神经调质或局部激素。
Endothelin (ET) is a recently identified vasoactive peptide with three isoforms for which three genes have been cloned. The cellular sites of synthesis of this peptide have not yet been identified in vivo. Using Northern blot analysis, we have detected two forms of ET mRNA in rat tissues: a 3.7-kilobase form in the kidney, eye, and brain, a 2.5-kilobase form in the intestine, and both forms in the lung. We have localized these forms of ET mRNA in several rat tissues using in situ hybridization. In the 19-day rat fetus, ET mRNA is highest in the lung, intestine, and meninges. At high resolution, ET mRNA is localized in the lung to respiratory epithelial cells of bronchioles and apparently in blood vessels. In adult tissues, ET mRNA is present throughout the lung, in the renal medulla vasa recta, and in the iris of the eye. ET mRNA is synthesized in close proximity to ET binding sites in many organs (e.g., lung, kidney, intestine, and eye), suggesting a local action of this peptide. However, in other areas (e.g., heart and renal cortex), ET binding sites are present in the absence of ET mRNA, suggesting an action of ET from the bloodstream or from neurons. Northern blot analysis of ET mRNA in microvascular endothelial cells in culture indicates that ET is synthesized in small blood vessels and regulated similarly to its regulation in large vessels. Our results provide evidence that ET, like other regulatory peptides, may serve in several tissues as a neuromodulator or local hormone.