The entire miR-200 seed family is strongly deregulated in clear cell renal cell cancer compared to the proximal tubular epithelial cells of the kidney
The entire miR-200 seed family is strongly deregulated in clear cell renal cell cancer compared to the proximal tubular epithelial cells of the kidney
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DOI：
10.1002/gcc.22016
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发表时间：
2013-02-01
影响因子：
3.7
通讯作者：
Kok, Klaas
中科院分区：
文献类型：
--
作者：
Duns, Gerben;van den Berg, Anke;Kok, Klaas
尽管许多研究报告了透明细胞肾细胞癌（ccRCC）中失调的microRNA（miRNA）和基因表达模式，但尚未与其假定的正常对应物：肾近端肾小管上皮细胞（PTECs）进行直接比较。本研究的目的是确定10个ccRCC衍生细胞系和PTEC短期培养物的miRNA表达谱，并将其与基因表达和拷贝数谱相关联。使用基于微阵列的方法，在ccRCC细胞系中观察到23种miRNA和1630种基因的表达水平显著改变。具有显著降低的表达水平的miRNA组包括已知参与上皮向间充质转化过程的miR-200家族的所有成员。47个经验证的下调miRNA靶基因中有13个的表达水平增加了两倍以上。我们的数据强化了上皮细胞向间充质细胞转化过程在ccRCC发展中的重要性。(c)2012 Wiley Periodicals，Inc.
Despite numerous studies reporting deregulated microRNA (miRNA) and gene expression patterns in clear cell renal cell carcinoma (ccRCC), no direct comparisons have been made to its presumed normal counterpart: the renal proximal tubular epithelial cells (PTECs). The aim of this study was to determine the miRNA expression profiles of 10 ccRCC-derived cell lines and short-term cultures of PTEC and to correlate these with their gene expression and copy-number profiles. Using microarray-based methods, a significantly altered expression level in ccRCC cell lines was observed for 23 miRNAs and 1630 genes. The set of miRNAs with significantly decreased expression levels include all members of the miR-200 family known to be involved in the epithelial to mesenchymal transition process. Expression levels of 13 of the 47 validated target genes for the downregulated miRNAs were increased more than twofold. Our data reinforce the importance of the epithelial to mesenchymal transition process in the development of ccRCC. (c) 2012 Wiley Periodicals, Inc.