Databases and Archiving for CryoEM.
Databases and Archiving for CryoEM.
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DOI：
10.1016/bs.mie.2016.04.015
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发表时间：
2016
影响因子：
--
通讯作者：
Lawson CL
中科院分区：
文献类型：
--
作者：
Patwardhan A;Lawson CL
结构生物学中的冷冻EM目前由三个公共档案 - 用于3DEM重建的EMDB，用于由3DEM重建构建的模型的PDB，以及用于获得3DEM重建的RAW 2D图像数据的empiar。结构社区和更广泛的生物社区使数据可访问，以便将结果重新使用，重新评估并与其他结构和生物信息学集成在一起资源的重要作用是由许多期刊授权数据对PDB和EMDB的授权进行的，该领域目前正在进行变革性的变化。另一方面，电子层析成像正在使大分子在细胞环境中进行研究。
在本章中，我们描述了档案的当前状态，可用于存入，访问，搜索，可视化和验证数据的资源，持续的社区范围内的计划以及未来的机会和挑战。
Cryo-EM in structural biology is currently served by three public archives – EMDB for 3DEM reconstructions, PDB for models built from 3DEM reconstructions and EMPIAR for the raw 2D image data used to obtain the 3DEM reconstructions. These archives play a vital role for both the structural community and the wider biological community in making the data accessible so that results may be reused, reassessed and integrated with other structural and bioinformatics resources. The important role of the archives is underpinned by the fact that many journals mandate the deposition of data to PDB and EMDB on publication. The field is currently undergoing transformative changes where on the one hand high-resolution structures are becoming a routine occurrence while on the other hand electron tomography is enabling the study of macromolecules in the cellular context. Concomitantly the archives are evolving to best serve their stakeholder communities. In this chapter we describe the current state of the archives, resources available for depositing, accessing, searching, visualising and validating data, on-going community-wide initiatives and opportunities and challenges for the future.