Dietary tryptophan requirement of fingerling Channa punctatus (Bloch) based on growth, hematological parameters, intestinal enzymes, non-specific immune response, and antioxidant capacity
Dietary tryptophan requirement of fingerling Channa punctatus (Bloch) based on growth, hematological parameters, intestinal enzymes, non-specific immune response, and antioxidant capacity
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DOI：
10.1016/j.aquaculture.2022.738745
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发表时间：
2022-08-23
期刊：
影响因子：
4.5
通讯作者：
Khan，Mukhtar A.
中科院分区：
文献类型：
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作者：
Sharf，Yusra;Khan，Mukhtar A.
在这项调查中，色氨酸对FC的要求。将色氨酸1、2、3、4、5、6和10 mg/kg−1分别饲喂7种等蛋白(450g/kg−1CP)和等能(14.7kJ g−1DE)日粮，随机分成3组，分别在接近饱和的8：00、12：30和17：30饲养12周，测定斑点仔鱼的产仔数(5.45±0.07)g。基础日粮中酪蛋白和明胶对色氨酸的贡献率为1 ng，为达到所需色氨酸水平，分别在基础日粮中添加结晶色氨酸1、1、2、3、4、5和9 mg/kg−。试验日粮中色氨酸的分析值分别为0.9、1.5、2.6、3.3、4.7、5.4和9.1g/kg−1，分别命名为Tr0.9、Tr1.5、Tr2.6、Tr3.3、Tr4.7、Tr5.4和Tr9.1，显著提高(P<；当色氨酸含量增加到4.7g/kg−1干日粮时，绝对增重、比生长率、蛋白质滞留率、蛋白质增重率和核糖核酸比值均显著高于对照组。屠体蛋白质和脂肪含量也显著提高，−1色氨酸含量达到4.7g/kg。日粮中−-1色氨酸含量为4.7g/kg时，对血细胞比容、血红蛋白、红细胞计数、血沉等血液学指标有正向影响(P<；P<0.05)。血清蛋白、谷胱甘肽过氧化物酶、超氧化物歧化酶、过氧化氢酶和溶菌酶也呈正相关，达4.7g/kg−1日粮色氨酸含量，而丙二醛、丙氨酸氨基转移酶和天冬氨酸氨基转移酶则相反。对日粮色氨酸含量进行二次回归分析，得出−-1日粮色氨酸需要量分别为5.43、5.5、5.21、4.96、4.81、4.85和5.39g/kg。根据上述参数，建议饲料中色氨酸的添加范围为4.81-5.5g/kg−1，以配制色氨酸平衡、实用的饲料。
In this investigation, the tryptophan requirement ofC. punctatusfingerling (5.45 ± 0.07 g) was determined by feeding seven isoproteic (450 g kg−1CP) and isoenergetic (14.7 kJ g−1DE) diets with graded levels of tryptophan 1, 2, 3, 4, 5, 6, and 10 g kg−1, respectively to randomly stocked groups in triplicate at 8:00, 12:30 and 17:30 h near to satiety for 12 weeks. Tryptophan contributed by casein and gelatin in basal diet was 1 g. To attain the desired tryptophan levels 0, 1, 2, 3, 4, 5 and 9 g kg−1crystalline tryptophan was added to basal diet. The analyzed value of tryptophan in the experimental diets was found to be 0.9, 1.5, 2.6, 3.3, 4.7, 5.4 and 9.1 g kg−1, and were designated as Tr0.9, Tr1.5, Tr2.6, Tr3.3, Tr4.7, Tr5.4and Tr9.1.Significant enhancement (P< 0.05) was found in Absolute weight gain (AWG), specific growth rate (SGR), protein retention efficiency (PRE), protein gain (PG), and RNA/DNA ratio with increasing tryptophan content up to 4.7 g kg−1dry diet. Carcass protein and fat also improved significantly up to 4.7 g kg−1tryptophan content. Hematological indices such as hematocrit, hemoglobin, red blood cell counts, erythrocyte sedimentation rate were positively affected (P< 0.05) up to 4.7 g kg−1tryptophan content of the diet. Serum protein, glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), and lysozyme were also positively correlated (P< 0.05) up to 4.7 g kg−1dietary tryptophan content while malondialdehyde (MDA), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) showed the inverse pattern. On subjecting the AWG, FCR, PRE, PG, RNA/DNA ratio, Hb and SOD against dietary tryptophan content to quadratic regression analysis, the tryptophan requirement was found at 5.43, 5.5, 5.21, 4.96, 4.81, 4.85 and 5.39 g kg−1diet, respectively. Based on the parameters mentioned above, the addition of tryptophan in feed in the range of 4.81–5.5 g kg−1is recommended for formulating tryptophan balanced, practical feeds for this fish.