Virtues and limitations of Pittsburgh green for ozone detection.
Virtues and limitations of Pittsburgh green for ozone detection.
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DOI：
10.1039/c4cc08244c
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发表时间：
2015-01
影响因子：
4.9
通讯作者：
Carlos Castelló Beltrán;Elliott A Palmer;B. Buckley;F. Iza
中科院分区：
文献类型：
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作者：
Carlos Castelló Beltrán;Elliott A Palmer;B. Buckley;F. Iza
最近提出的用于检测臭氧的2 '，7'-二氯荧光素（DCF）衍生的荧光探针显示出对许多活性氧的良好选择性和良好的pH稳定性，用于生物和环境应用。然而，发现可能发生荧光产物（匹兹堡绿色）的过度氧化。这可能导致定量测量不准确，因为过度氧化副产物的荧光和重叠荧光信号减少，需要仔细的实验设计。虽然难以通过荧光测量来评估，但可以通过（1）H NMR光谱法方便地监测过氧化。
A recently proposed 2',7'-dichlorofluorescein (DCF)-derived fluorescent probe for the detection of ozone shows good selectivity against a number of reactive oxygen species and good pH stability for biological and environmental applications. It is found, however, that over oxidation of the fluorescent product (Pittsburgh green) can occur. This could render quantitative measurements inaccurate due to a reduction in fluorescence and overlapping fluorescence signals from over oxidation by-products and it requires careful experimental design. Although difficult to assess by fluorescence measurements, the over oxidation can be conveniently monitored by (1)H NMR spectroscopy.