Developmentally excised sequences in micronuclear DNA of Paramecium.
Developmentally excised sequences in micronuclear DNA of Paramecium.
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DOI：
10.1073/pnas.91.6.2255
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发表时间：
1994-03
影响因子：
11.1
通讯作者：
C. J. Steele;G. A. Barkocy-Gallagher;L. B. Preer;J. Preer
中科院分区：
文献类型：
--
作者：
C. J. Steele;G. A. Barkocy-Gallagher;L. B. Preer;J. Preer
DNA加工发生在纤毛虫的自交配和结合过程中，微核形成新的大核，旧的大核降解。微核DNA的加工包括去除某些内部序列、染色体断裂、添加新的端粒和扩增。除了最近的一份简短报告外，在草履虫中没有描述内部消除序列。在本文中，我们描述了在四甲草履虫编码表面蛋白A的基因内部和附近发现的九个内部消除序列。在这9个序列中，有7个位于基因的翻译部分，并且都包含短的反向末端重复序列。特征序列TA出现在所有内部消除序列的边界处。
DNA processing occurs in ciliates at autogamy and conjugation when new macronuclei are formed from micronuclei and old macronuclei degrade. Processing of micronuclear DNA consists of removal of certain internal sequences, chromosomal fragmentation, addition of new telomeres, and amplification. Aside from a recent brief report, internal eliminated sequences have not been described in Paramecium. In this paper we characterize nine internal eliminated sequences found within and near the gene that codes for surface protein A in Paramecium tetraurelia. Of these nine, seven are located within the translated portion of the gene, and all include short, inverted terminal repeats. The characteristic sequence, TA, appears at the boundaries of all of the internal eliminated sequences.