A Laotian strain of the rotifer Brachionus angularis holds promise as a food source for small-mouthed larvae of freshwater fish in aquaculture
A Laotian strain of the rotifer Brachionus angularis holds promise as a food source for small-mouthed larvae of freshwater fish in aquaculture
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DOI：
10.1016/j.aquaculture.2010.12.017
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发表时间：
2011-02-25
期刊：
影响因子：
4.5
通讯作者：
Kurokura, Hisashi
中科院分区：
文献类型：
--
作者：
Ogata, Yuka;Tokue, Yuri;Kurokura, Hisashi
在老挝万象分离到一种非常小的淡水轮虫角臂轮虫（老挝株）。我们研究了该菌株的形态、放牧和生长特性。老挝菌株的lorica为圆形，长度86.0 +/- 4.9 μ m（平均+/- SD），有少量小刺。这些形态特征使该菌株成为淡水鱼小口幼虫的适宜活食。轮虫对小球藻的掠食率以密度依赖的方式增加，最高可达约10 × 106个藻类细胞ml（-1）。在0.5-10 × 10(6)个海藻细胞ml（-1）时，轮虫的过滤率稳定(约100 μ l /个体/小时[ind.(-1) h(-1)])，在较高的普通螺旋体密度(20 × 10（6)个细胞ml(-1)）时，轮虫的过滤率下降到30-50 μ l /个体/小时（-1）h（-1）。在批量培养中，当投喂7 × 10(6)和10 × 10(6)个细胞ml（-1）时，轮虫菌株在24℃和27℃条件下生长良好；在此条件下，轮虫密度在10天内达到了100 000 ind.ml（-1）。用个体培养法获得生活史参数。温度越高、藻密度越高，生成时间越短。(C) 2010 Elsevier B.V.版权所有
A very small strain of the freshwater rotifer Brachionus angularis (Laos strain) was isolated in Vientiane, Laos. We investigated the morphology as well as grazing and growth characteristics of this strain. The lorica of the Laos strain was round, 86.0 +/- 4.9 mu m (mean +/- SD) in length, with a few small spines. These morphological characteristics make the strain an appropriate live food for small-mouthed larvae of freshwater fish. The rotifer's grazing rate on Chlorella vulgaris increased in a density-dependent manner up to approximately 10 x 106 algal cells ml(-1). The rotifer filtering rate was stable (ca. 100 mu l per individual per hour [ind.(-1) h(-1)]) at 0.5-10 x 10(6) algal cells ml(-1) and declined to 30-50 mu l ind.(-1) h(-1) at a higher C. vulgaris density (20 x 10(6) cells ml(-1)). In batch cultures, the rotifer strain grew well at 24 degrees C and 27 degrees C when fed with algal densities of 7 x 10(6) and 10 x 10(6) cells ml(-1); under these conditions, the rotifer density reached > 2000 ind.ml(-1) within 10 days. Life history parameters were obtained using the individual culture method. The generation time became shorter at higher temperatures and higher algal densities. (C) 2010 Elsevier B.V. All rights reserved.