Palladin is expressed preferentially in excitatory terminals in the rat central nervous system
Palladin is expressed preferentially in excitatory terminals in the rat central nervous system
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DOI：
10.1002/cne.1062.abs
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发表时间：
2001-07-23
影响因子：
2.5
通讯作者：
Valtschanoff, JG
中科院分区：
文献类型：
--
作者：
Hwang, SJ;Pagliardini, S;Valtschanoff, JG
Palladin是最近发现的一种与成纤维细胞中肌动蛋白细胞骨架和细胞粘附相关的细胞内蛋白。在Western和北方印迹分析中，palladin表达在胚胎小鼠中普遍存在，但在大多数成年组织中显著下调。通过Western印迹分析评估，大量的palladin持续存在于成年啮齿动物的大脑中。通过这项工作，我们扩展了初步的观察，并确定了palladin在整个大鼠大脑中的总体分布和亚细胞位置。矢状位。和中枢神经系统的冠状切片，palladin的免疫染色存在于整个脑和脊髓中，但不是均匀的。免疫染色的阳性区域包括嗅球、大脑和小脑皮质、海马、杏仁核、上级丘和脊髓背角的浅层。因为免疫染色的特征是点状的，我们对palladin和突触前标记物突触素进行了双重染色。共聚焦显微镜显示，palladin免疫阳性斑点也对突触体素免疫阳性;也对palladin染色的突触体素免疫阳性斑点的比例范围从海马CA 3区和小脑皮质中100%的苔藓纤维末端到大脑皮质、纹状体和脊髓背角中60-70%的末端。电子显微镜证实了突触终末中palladin的存在。因为免疫染色的末端通常建立不对称突触，所以通过对palladin和γ-氨基丁酸的双重染色来测试突触末端中palladin表达的选择性。在光镜和电子显微镜水平上，这种材料中的适度共定位水平表明palladin对释放兴奋性神经递质的终端具有选择性。作为伴随的工作在细胞培养中已经表明，palladin参与轴突的发育和迁移，目前的结果表明，palladin坚持在兴奋性突触的成年神经系统。神经学比较杂志436：211-224，2001. (C)2001 Wiley-Liss，Inc.
Palladin is a recently described intracellular protein associated with the actin cytoskeleton and cell adhesion in fibroblasts. In Western and Northern blot analyses, palladin expression is ubiquitous in embryonic mice, but it is down-regulated dramatically in most adult tissues. Significant amounts of palladin persist in the brain of adult rodents, as assessed by Western blot analysis. With this work, we extend preliminary observations and determine the overall distribution and subcellular location of palladin throughout the rat brain. In sagittal. and coronal sections of the central nervous system, immunostain for palladin is present throughout the brain and spinal cord, but not uniformly. The densest regions of immunostain include the olfactory bulb, cerebral and cerebellar cortex, hippocampus, amygdala, superior colliculus, and superficial laminae of the spinal dorsal horn. Because immunostain characteristically is punctate, we performed double staining for palladin and the presynaptic marker synaptophysin. Confocal microscopy showed that palladin-immunopositive puncta are also immunopositive for synaptophysin; the proportion of synaptophysin-immunopositive puncta that also stained for palladin ranged from 100% of mossy fiber terminals in field CA3 of the hippocampus and in the cerebellar cortex to 60-70% of terminals in the cerebral cortex, striatum, and spinal dorsal horn. The presence of palladin in synaptic terminals was confirmed by electron microscopy. Because immunostained terminals commonly establish asymmetric synapses, the selectivity of palladin expression in synaptic terminals was tested by double staining for palladin and gamma -aminobutyric acid. The modest level of colocalization in this material at both the light microscopic and electron microscopic levels suggests a selectivity of palladin for terminals that release excitatory neurotransmitters. As concomitant work in cell cultures has shown that palladin participates in axonal development and migration, the present results suggest that palladin persists at excitatory synapses of the adult nervous system. J. Comp. Neurol. 436:211-224, 2001. (C) 2001 Wiley-Liss, Inc.