Atherogenic high-fat diet reduces bone mineralization in mice
Atherogenic high-fat diet reduces bone mineralization in mice
复制标题
DOI：
10.1359/jbmr.2001.16.1.182
复制
发表时间：
2001-01-01
影响因子：
6.2
通讯作者：
Demer, LL
中科院分区：
文献类型：
--
作者：
Parhami, F;Tintut, Y;Demer, LL
骨质疏松症与心血管疾病之间的流行病学相关性与年龄无关，但这种相关性的基础尚不清楚。我们之前发现致动脉粥样硬化的氧化脂质在体外和离体抑制成骨细胞分化，这表明致动脉粥样硬化饮食可能导致这两种疾病。在这项研究中，在对动脉粥样硬化和脂质氧化具有不同遗传易感性的两种小鼠品系中，测试了致动脉粥样硬化高脂肪饮食与对照饮食对骨骼的影响。在饮食4个月和7个月后，使用外周定量计算机断层扫描（pQCT）测量了切除的股骨和腰椎中的矿物质含量和密度。此外，对饮食4个月后从小鼠分离的骨髓中骨钙素的表达进行了检查。 7个月后，与普通饮食的小鼠相比，接受高脂饮食的C57BL/6动脉粥样硬化易感小鼠的股骨矿物质含量降低了43%（0.73 +/- 0.09 mg vs. 1.28 +/- 0.42 mg；p = 0.008），并且矿物质密度降低了15%。 4 个月后观察到较小的赤字。脂肪喂养的 C57BL/6 小鼠的椎骨矿物质含量也较低。抗动脉粥样硬化的 C3H/HeJ 小鼠中的这些变化较小，而且大多不显着。高脂肪喂养的 C57BL/6 小鼠的骨髓中骨钙素表达降低。这些发现表明，致动脉粥样硬化饮食通过阻止成骨祖细胞的分化来抑制骨形成。
The epidemiological correlation between osteoporosis and cardiovascular disease is independent of age, but the basis for this correlation is unknown. We previously found that atherogenic oxidized lipids inhibit osteoblastic differentiation in vitro and ex vivo, suggesting that an atherogenic diet may contribute to both diseases. In this study, effects of an atherogenic high-fat diet versus control chow diet on bone were tested in two strains of mice with genetically different susceptibility to atherosclerosis and lipid oxidation, After 4 months and 7 months on the diets, mineral content and density were measured in excised femurs and lumbar vertebrae using peripheral quantitative computed tomographic (pQCT) scanning. In addition, expression of osteocalcin in marrow isolated from the mice after 4 months on the diets was examined. After 7 months, femoral mineral content in C57BL/6 atherosclerosis-susceptible mice on the high-fat diet was 43% lower (0.73 +/- 0.09 mg vs. 1.28 +/- 0.42 mg; p = 0.008), and mineral density was 15% lower compared with mice on the chow diet. Smaller deficits were observed after 4 months. Vertebral mineral content also was lower in the fat-fed C57BL/6 mice. These changes in the atherosclerosis-resistant, C3H/HeJ mice were smaller and mostly not significant. Osteocalcin expression was reduced in the marrow of high fat-fed C57BL/6 mice, These findings suggest that an atherogenic diet inhibits bone formation by blocking differentiation of osteoblast progenitor cells.