DIRECT LIQUID-CHROMATOGRAPHIC SEPARATION OF ENANTIOMERS ON IMMOBILIZED PROTEIN STATIONARY PHASES .9. INFLUENCE OF THE CROSS-LINKING REAGENT ON THE RETENTIVE AND ENANTIOSELECTIVE PROPERTIES OF CHIRAL SORBENTS BASED ON BOVINE SERUM-ALBUMIN
DIRECT LIQUID-CHROMATOGRAPHIC SEPARATION OF ENANTIOMERS ON IMMOBILIZED PROTEIN STATIONARY PHASES .9. INFLUENCE OF THE CROSS-LINKING REAGENT ON THE RETENTIVE AND ENANTIOSELECTIVE PROPERTIES OF CHIRAL SORBENTS BASED ON BOVINE SERUM-ALBUMIN
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DOI：
10.1016/0021-9673(92)80223-h
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发表时间：
1992-02-07
期刊：
影响因子：
--
通讯作者：
ALLENMARK, SG
中科院分区：
文献类型：
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作者：
ANDERSSON, S;THOMPSON, RA;ALLENMARK, SG
评价了三种硅胶结合的交联型牛血清白蛋白(BSA)作为手性吸附剂用于对映体的高效液相色谱分离。以戊二醛、甲醛和二(N-琥珀酰亚胺)碳酸酯为双功能试剂对牛血清白蛋白进行固定化。所有的吸附剂都含有相同的BSA负载量(14.4+/-0.1%，w/w)，并且仅在用于固定化的交联剂方面有所不同。尽管它们表面上相似，但它们的层析性能非常不同，不仅在保留被分析物对映体(k‘和α)方面，而且在柱效方面(影响R(S)值)。所得数据表明，交联剂的化学结构在很大程度上影响重要的手性结合部位的可及性。虽然所获得的数据很难详细解释，但可以对吸附剂的不同行为作出某些概括。
Three modifications of silica-bound, cross-linked bovine serum albumin (BSA) were evaluated as chiral sorbents for use in the liquid chromatographic separation of enantiomers. Glutaraldehyde, formaldehyde and di-(N-succinimidyl) carbonate were used as bifunctional reagents for the immobilization of BSA. The sorbents all contain the same loading of BSA (14.4 +/- 0.1%, w/w) and differ only with respect to the cross-linker used for immobilization. Despite their apparent similarity, the sorbents show very different chromatographic properties, not only with respect to retention of analyte enantiomers (k' and alpha), but also with respect to column efficiency (affecting R(s) values). The data obtained indicate that the chemical structure of the cross-linking reagent affects to a large extent the accessibility of important chiral binding sites. Although the data obtained are difficult to interpret in any detail, certain generalizations concerning the different behaviour of the sorbents can be made.