Calmodulin activates prokaryotic adenylate cyclase.
Calmodulin activates prokaryotic adenylate cyclase.
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钙调蛋白激活原核腺苷酸环化酶。
DOI：
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发表时间：
1980
影响因子：
11.1
通讯作者：
S. Berkowitz
中科院分区：
文献类型：
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作者：
J. Wolff;G. H. Cook;A. Goldhammer;S. Berkowitz
百日咳博德特氏菌的腺苷酸环化酶被小牛脑钙调素以剂量依赖性方式刺激100至1000倍。该系统具有以下属性。(i)乙二醇双（β-氨基乙基醚）-N，N，N '，N'-四乙酸可阻止活化，Ca ~（2+）可恢复活化。(ii)钙调蛋白的甲硫氨酸残基的氧化消除了激活环化酶的能力。(iii)三氟拉嗪抑制钙调素激活的环化酶。(iv)肌钙蛋白C制剂刺激B。百日咳环化酶与钙调素的效价< 0.01。虽然钙调蛋白尚未在原核生物中得到证实，但这是原核生物中（真核）钙调蛋白效应的一个例子。
The adenylate cyclase of Bordetella pertussis is stimulated 100- to 1000-fold in a dose-dependent manner by calf brain calmodulin. The system has the following properties. (i) The activation is prevented by ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid and restored by Ca2+. (ii) Oxidation of the methionine residues of calmodulin abolishes the ability to activate the cyclase. (iii) Trifluoperazine inhibits calmodulin-activated cyclase. (iv) A troponin C preparation stimulates the B. pertussis cyclase with < 0.01 the potency of calmodulin. Although calmodulin has not been demonstrated in prokaryotes, this is an example of a (eukaryotic) calmodulin effect in a prokaryote.