Exposure to Sevoflurane Affects the Development of Parvalbumin Interneurons in the Main Olfactory Bulb in Mice.
Exposure to Sevoflurane Affects the Development of Parvalbumin Interneurons in the Main Olfactory Bulb in Mice.
复制标题
暴露于七氟醚会影响小鼠主嗅球中小白蛋白中间神经元的发育
DOI：
10.3389/fnana.2016.00072
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发表时间：
2016
影响因子：
2.9
通讯作者：
Ling S
中科院分区：
文献类型：
--
作者：
Yang J;Chen J;Cai G;Lu R;Sun T;Luo T;Wu S;Ling S
七氟烷广泛用于成人和儿童患者的临床手术。尽管研究表明，手术后暴露于七氟烷会损害嗅觉记忆，但这种影响背后的神经病理学变化尚不清楚。本研究检测了七氟烷暴露对主嗅球（MOB）中表达钙结合蛋白的中间神经元发育的影响。我们在两个不同的发育时间点将新生小鼠暴露于2%七氟烷，发现在出生后（PD）7天将小鼠暴露于七氟烷显著降低了嗅球（OB）中GAD 67和小白蛋白（PV）的表达，但没有改变钙视网膜蛋白（CR）或钙结合蛋白D28k（CB）的表达。在PD 7时，MOB中PV表达中间神经元的数量和树突形态因暴露于七氟烷而受损。然而，在PD 10时暴露于七氟烷对MOB中的钙结合蛋白表达或PV表达中间神经元的数量和树突形态没有影响。这些结果表明，在嗅觉发育的关键时期将新生小鼠暴露于七氟烷会影响MOB中PV表达中间神经元的发育。
Sevoflurane is widely used in adult and pediatric patients during clinical surgeries. Although studies have shown that exposure to sevoflurane impairs solfactory memory after an operation, the neuropathological changes underlying this effect are not clear. This study detected the effect of sevoflurane exposure on the development of calcium-binding proteins-expressing interneurons in the main olfactory bulb (MOB). We exposed neonatal mice to 2% sevoflurane at two different developmental time points and found that exposing mice to sevoflurane at postnatal day (PD) 7 significantly decreased the expression of GAD67 and parvalbumin (PV) in the olfactory bulb (OB) but did not alter the expression of calretinin (CR) or calbindin D28k (CB). The number and dendritic morphology of PV-expressing interneurons in the MOB were impaired by exposure to sevoflurane at PD7. However, exposure to sevoflurane at PD10 had no effect on calcium-binding protein expression or the number and dendritic morphology of PV-expressing interneurons in the MOB. These results suggest that exposing neonatal mice to sevoflurane during a critical period of olfactory development affects the development of PV-expressing interneurons in the MOB.