Resonant Acoustic Rheometry to Measure Coagulation Kinetics in Hemophilia A and Healthy Plasma: A Novel Viscoelastic Method
Resonant Acoustic Rheometry to Measure Coagulation Kinetics in Hemophilia A and Healthy Plasma: A Novel Viscoelastic Method
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DOI：
10.1055/s-0042-1757896
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发表时间：
2022-11-01
影响因子：
5.7
通讯作者：
Deng, Cheri X.
中科院分区：
文献类型：
--
作者：
Li, Weiping;Hobson, Eric C.;Deng, Cheri X.
与传统的凝血试验和因子特异性分析相比，粘弹性止血试验(VHAs)可以更全面地评估血栓的形成和溶解，但也有一些局限性，包括血栓变形。在这项概念验证研究中，我们测试了一种名为共振声流变仪(RAR)的非接触式技术，用于测量人体血浆凝固的动力学。具体地说，RAR利用双模超声技术在没有直接物理接触的情况下诱导和检测血液样本的表面振荡，并测量随着时间的推移表面振荡的共振频率，这反映了样本的粘弹性。对正常血浆RAR结果的分析允许定义一组用于量化凝血的参数。RAR在血友病A血浆中检测到共振频率的平坦轨迹，并通过添加组织因子进行了校正。我们的RAR结果捕捉到了血浆凝血的动力学和新定义的RAR参数，这些参数与健康和血友病A血浆中组织因子浓度的增加相关。这些发现证明了RAR作为VHA的一种新方法的可行性，为未来比较RAR参数与常规凝血试验、因子特异性分析和VHA参数提供了基础。
Compared with conventional coagulation tests and factor-specific assays, viscoelastic hemostatic assays (VHAs) can provide a more thorough evaluation of clot formation and lysis but have several limitations including clot deformation. In this proof-of-concept study, we test a noncontact technique, termed resonant acoustic rheometry (RAR), for measuring the kinetics of human plasma coagulation. Specifically, RAR utilizes a dual-mode ultrasound technique to induce and detect surface oscillation of blood samples without direct physical contact and measures the resonant frequency of the surface oscillation over time, which is reflective of the viscoelasticity of the sample. Analysis of RAR results of normal plasma allowed defining a set of parameters for quantifying coagulation. RAR detected a flat-line tracing of resonant frequency in hemophilia A plasma that was corrected with the addition of tissue factor. Our RAR results captured the kinetics of plasma coagulation and the newly defined RAR parameters correlated with increasing tissue factor concentration in both healthy and hemophilia A plasma. These findings demonstrate the feasibility of RAR as a novel approach for VHA, providing the foundation for future studies to compare RAR parameters to conventional coagulation tests, factor-specific assays, and VHA parameters.