GR1 and NTRA involved in pollen tube growth in the stigma of Arabidopsis
GR1 and NTRA involved in pollen tube growth in the stigma of Arabidopsis
复制标题
DOI：
10.1007/s00425-023-04161-9
复制
发表时间：
2023-05
期刊：
影响因子：
4.3
通讯作者：
M. Zhang;Jing Jing Cui-Jing;Zi Ming Wang;Y. Dong;Xin-Qi Gao
中科院分区：
文献类型：
--
作者：
M. Zhang;Jing Jing Cui-Jing;Zi Ming Wang;Y. Dong;Xin-Qi Gao
主要结论拟南芥GR 1和NTRA在授粉过程中参与花粉管穿透柱头进入传递道的过程，在授粉过程中，花粉（管）与柱头之间的识别介导了花粉的水合和萌发，以及花粉管在柱头上的生长，拟南芥谷胱甘肽还原酶1（GR 1）和NADPH依赖的硫氧还蛋白还原酶A（NTRA）参与调节细胞氧化还原止血。GR 1和NTRA在花粉中均有表达，但它们在花粉萌发和花粉管生长中的作用有待进一步研究。在本研究中，我们进行了授粉实验，发现拟南芥gr 1/+ntra/-和gr 1/- ntra/+双突变损害了雄配子体的传递。突变体花粉形态和生活力未见明显异常。此外，双突变体的花粉在固体花粉萌发培养基上的水合和萌发与野生型相当。而gr 1突变体的花粉管在柱头表面生长时，不能穿透柱头进入传递道。以上结果表明，GR 1和NTRA在授粉过程中对花粉管与柱头之间的相互作用起调节作用。
Main conclusionArabidopsis GR1 and NTRA function in pollen tube penetrating the stigma into the transmitting tract during pollination.AbstractDuring pollination, recognition between pollen (tube) and stigma mediates the hydration and germination of pollen, as well as the growth of the pollen tube on the stigma.Arabidopsisglutathione reductase 1 (GR1) and NADPH-dependent thioredoxin reductase A (NTRA) are involved in regulating cell redox hemostasis. BothGR1andNTRAare expressed in pollen, but their roles in pollen germination and the growth of the pollen tube need further investigation. In this study, we performed pollination experiments and found that theArabidopsis gr1/+ntra/–andgr1/– ntra/+ double mutation compromised the transmission of male gametophytes. Pollen morphology and viability of the mutants did not show obvious abnormalities. Additionally, the pollen hydration and germination of the double mutants on solid pollen germination medium were comparable to those of the wild type. However, the pollen tubes withgr1 ntradouble mutation were unable to penetrate the stigma and enter the transmitting tract when they grew on the surface of the stigma. Our results indicate thatGR1andNTRAplay a role in regulating the interaction between the pollen tube and the stigma during pollination.