Shear-Induced Self-Assembly of Native Silk Proteins into Fibrils Studied by Atomic Force Microscopy
Shear-Induced Self-Assembly of Native Silk Proteins into Fibrils Studied by Atomic Force Microscopy
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DOI：
10.1021/bm201509b
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发表时间：
2012-03-01
影响因子：
6.2
通讯作者：
Schniepp, Hannes C.
中科院分区：
文献类型：
--
作者：
Greving, Imke;Cai, Minzhen;Schniepp, Hannes C.
采用非接触模式原子力显微镜对不同制备方法制备的天然丝蛋白进行了研究。低蛋白浓度下，单个蛋白分子呈简单的圆形，表观直径为20-25 nm。从腺体中提取后，在干燥前剪切天然蛋白质溶液，形成纳米级的串珠组装。蛋白质浓度对蛋白质组合的形态有显著影响。在较高的蛋白质浓度下，观察到剪切诱导排列成纳米原纤维，而较低的浓度导致形成更薄的原纤维，宽度约为8 nm。
Noncontact mode atomic force microscopy was used to investigate native silk proteins prepared in different ways. Low protein concentrations revealed that single protein molecules exhibit a simple, round shape with apparent diameters of 20-25 nm. Shearing the native protein solutions after extraction from the gland and prior to drying led to a beads-on-a-string assembly at the nanometer scale. Protein concentration had a significant effect on the morphology of the protein assemblies. At higher protein concentrations, shear-induced alignment into nanofibrils was observed, while lower concentrations lead to the formation of much thinner fibrils with a width of about 8 nm.