Design and synthesis study of the thermo-sensitive copolymer carrier of penicillin G acylase
Design and synthesis study of the thermo-sensitive copolymer carrier of penicillin G acylase
复制标题
青霉素G酰化酶热敏共聚物载体的设计与合成研究
DOI：
10.1002/pat.4299
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发表时间：
2018-07-01
影响因子：
3.4
通讯作者：
Liu, Zhen
中科院分区：
文献类型：
--
作者：
Li, Ke;Chen, Zhen Bin;Liu, Zhen
本研究采用可逆-加成-断裂链转移聚合方法制备了一系列温敏性三嵌段共聚物载体聚(N，N-二乙基丙烯酰胺-b-甲基丙烯酸羟乙酯-b-缩水甘油酯)、PDEA-b-PHEMA-b-PGMA(简称DHG)。通过核磁共振、凝胶渗透色谱、荧光光谱等手段进一步表征了它们的结构和温敏性能。结果表明，三嵌段共聚物的合成反应为活性聚合。热敏性研究表明，甲基丙烯酸羟乙酯和甲基丙烯酸缩水甘油酯两种单体对低临界溶液温度性能起着关键作用。最后，将上述嵌段共聚物用于固定化青霉素G酰化酶，考察了2种单体甲基丙烯酸羟乙酯和甲基丙烯酸缩水甘油酯的数均摩尔质量与固定化PGA的负载量(L)和活性回收率(Ar)之间的关系。结果表明，L和Ar分别达到了19502U和92%。
Reversible-addition-fragmentation chain transfer polymerization method was adopted here in this study to prepare a series of thermo-sensitive tri-block copolymer carrier, poly(N,N-diethylacrylamide-b--hydroxyethyl methacrylate-b-glycidyl methacrylate), PDEA-b-PHEMA-b-PGMA (named as DHG). Their structures and temperature sensitivity performances were further characterized by the nuclear magnetic resonance, the gel permeation chromatography, and the fluorescence spectroscopy, respectively. It has been identified that the synthesis reaction of tri-block copolymer was living polymerization. The thermo-sensitivity study suggested that 2 monomers, -hydroxyethyl methacrylate and glycidyl methacrylate, played key roles on the lower critical solution temperature performance. Finally, above block copolymer was conscripted to immobilize penicillin G acylase; relationships among the number-average molar weight of the 2 monomer, -hydroxyethyl methacrylate and glycidyl methacrylate, loading (L), and activity recover rate (Ar) of immobilized PGA were investigated. And the results showed that L and Ar arrived at 19502U and 92%, respectively.