Quorum sensing controls biofilm formation in Vibrio cholerae
Quorum sensing controls biofilm formation in Vibrio cholerae
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DOI：
10.1046/j.1365-2958.2003.03688.x
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发表时间：
2003-10-01
影响因子：
3.6
通讯作者：
Bassler, BL
中科院分区：
文献类型：
--
作者：
Hammer, BK;Bassler, BL
多个群体感应回路并行作用以控制霍乱弧菌的毒力和生物膜形成。与在存在群体感应自诱导物的情况下在高细胞密度下诱导毒力因子产生和/或生物膜形成的其他细菌病原体相反，霍乱弧菌在高细胞密度下抑制这些行为。与此一致，我们在此表明，“锁定”在模拟低细胞密度的调节状态的霍乱弧菌菌株对于生物膜产生是增强的，而“锁定”在模拟高细胞密度的调节状态的突变体不能产生生物膜。我们在霍乱弧菌中鉴定的群体感应级联调节参与胞外多糖产生（EPS）的基因的转录，并且产生EPS和形成生物膜的变体以高频率从非EPS、非生物膜产生菌株中出现。我们的数据表明，自发突变的转录调节hapR是负责这种效果。霍乱弧菌的几种致突变菌株在hapR中具有天然存在的移码突变。因此，这种水生病原体所占据的独特环境可能包括细胞间通讯至关重要的小生境，以及通过hapR突变丧失群体感应的小生境。细菌生物膜可能代表了一个复杂的栖息地，这种分化发生。
Multiple quorum-sensing circuits function in parallel to control virulence and biofilm formation in Vibrio cholerae. In contrast to other bacterial pathogens that induce virulence factor production and/or biofilm formation at high cell density in the presence of quorum-sensing autoinducers, V. cholerae represses these behaviours at high cell density. Consistent with this, we show here that V. cholerae strains 'locked' in the regulatory state mimicking low cell density are enhanced for biofilm production whereas mutants 'locked' in the regulatory state mimicking high cell density are incapable of producing biofilms. The quorum-sensing cascade we have identified in V. cholerae regulates the transcription of genes involved in exopolysaccharide production (EPS), and variants that produce EPS and form biofilms arise at high frequency from non-EPS, non-biofilm producing strains. Our data show that spontaneous mutation of the transcriptional regulator hapR is responsible for this effect. Several toxigenic strains of V. cholerae possess a naturally occurring frameshift mutation in hapR. Thus, the distinct environments occupied by this aquatic pathogen presumably include niches where cell-cell communication is crucial, as well as ones where loss of quorum sensing via hapR mutation confers a selective advantage. Bacterial biofilms could represent a complex habitat where such differentiation occurs.