Cyclical interactions between two outer doublet microtubules in split flagellar axonemes
Cyclical interactions between two outer doublet microtubules in split flagellar axonemes
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DOI：
10.1529/biophysj.105.067876
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发表时间：
2005-11-01
影响因子：
3.4
通讯作者：
Kamiya, R
中科院分区：
文献类型：
--
作者：
Aoyama, S;Kamiya, R
纤毛和鞭毛的跳动是基于相邻的外层双微管之间的局部滑动；然而，产生振荡弯曲的机制尚不清楚。为了阐明这一机制，我们利用高速录像技术对衣藻轴突磨损的行为进行了研究。一对双态微管在ATP的存在下经常表现出结合和解离循环。在许多情况下，两个微管的解离并不伴随着明显的弯曲，这表明动力蛋白与微管的相互作用不一定受微管曲率的调节。在极少数情况下，在同一相互作用对中同时发生联想和解离，导致对之间的一段间隙的尖端定向运动。基于这些观察，我们提出了轴突中周期性弯曲传播的模型。
The beating of cilia and flagella is based on the localized sliding between adjacent outer doublet microtubules; however, the mechanism that produces oscillatory bending is unclear. To elucidate this mechanism, we examined the behavior of frayed axonemes of Chlamydomonas by using high-speed video recording. A pair of doublet microtubules frequently displayed association and dissociation cycles in the presence of ATP. In many instances, the dissociation of two microtubules was not accompanied by noticeable bending, suggesting that the dynein-microtubule interaction is not necessarily regulated by the microtubule curvature. On rare occasions, association and dissociation occurred simultaneously in the same interacting pair, resulting in a tip-directed movement of a stretch of gap between the pair. Based on these observations, we propose a model for cyclical bend propagation in the axoneme.