Hypotension-induced expression of the c-fos gene in the medulla oblongata of piglets.
Hypotension-induced expression of the c-fos gene in the medulla oblongata of piglets.
复制标题
低血压诱导仔猪延髓中 c-fos 基因的表达。
DOI：
10.1016/0006-8993(95)01173-0
复制
发表时间：
1996
期刊：
影响因子：
2.9
通讯作者：
Gootman,PM
中科院分区：
文献类型：
--
作者：
Ruggiero,DA;Tong,S;Anwar,M;Gootman,N;Gootman,PM
神经网络介导压力感受器戒断反应在Sus scrofa。c-fos的诱导被用作监测周围血管扩张剂硝普钠维持的低血压的突触活动。比较藏红花麻醉的实验动物和年龄匹配的正常血压对照组的c-fos基因表达模式。控制其他变量的影响，包括术前1小时适应新环境、麻醉、血气和ph。相同的刺激后生存期允许转录物积累。用兔抗血清分别对c-fos蛋白的1-131氨基酸和4-17残基进行免疫细胞化学鉴定。在酪氨酸羟化酶（TH）抗血清标记的儿茶酚胺能神经元中鉴定出Fos。在低血压仔猪孤立束核（NTS）中诱导Fos。编码Fos的神经元与一级内脏传入的投射模式相匹配。与压力感受器场一致的背外侧核有明显的诱导作用。在其他压力感受器末端，如内侧亚核、内侧联合野、中间亚核和腹侧A2去肾上腺素能区，神经元活动指数明显增加。在网状结构中，c-fos蛋白在外侧被盖场（LTF）的限定柱中被诱导，从面核延伸到笔菖蒲。儿茶酚胺能（th阳性）神经元在猪腹外侧髓质C1区和A1区表达Fos。在LTF的背侧中间网状区也可诱导Fos。实验组和对照组在中缝苍白核、吻侧旁脉网状结构、上胸椎中外侧细胞柱和星状神经节上观察到轻微或不一致的差异。总之，压力感受器戒断在幼龄动物沿已建立的心血管反射通路诱导神经元反应模式。
Neural networks that mediate the reflex response to baroreceptor withdrawal were explored in Sus scrofa. Induction of c-fos was used as a monitor of synaptic activity in response to hypotension sustained by systemic administration of a peripheral vasodilator, sodium nitroprusside. Patterns of c-fos gene expression were compared between Saffan-anesthetized experimental animals and age-matched normotensive controls administered vehicle. Effects of other variables were controlled including 1 h preoperative accommodation to the novel environment, anesthesia, blood gases and pH. Identical post-stimulus survival periods were allowed for accumulation of transcript. The c-fos protein, Fos, was identified immunocytochemically with two rabbit antisera raised against amino acids 1–131 of Fos or residues 4–17 of synthetic human transcript. Fos was identified in catecholaminergic neurons labeled with an antiserum to tyrosine hydroxylase (TH). Fos was induced in the nucleus tractus solitarii (NTS) of hypotensive piglets. Neurons encoding Fos matched projection patterns of first order visceral afferents. Induction was prominent in the dorsolateral nucleus coinciding with the baroreceptor field. Indices of increased neuronal activity were evident in other baroreceptor terminal sites, e.g., medial subnucleus, the medial commissural field, the intermediate subnucleus and a ventral A2 noradrenergic area. In reticular formation c-fos protein was induced in circumscribed columns in the lateral tegmental field (LTF) extending from facial nucleus to calamus scriptorius. Catecholaminergic (TH-positive) neurons expressed Fos in the porcine C1 and A1 areas of ventrolateral medulla. Fos was also induced in a dorsal intermediate reticular zone of LTF. Minor or inconsistent differences between experimental and control were observed in nucleus raphe pallidus, rostral paramedian reticular formation, upper thoracic intermediolateral cell column, and stellate ganglia. In conclusion, baroreceptor withdrawal in young animals induced patterns of neuronal response along established cardiovascular reflex pathways.