Chimerism of Kobayashi-mikan (Citrus natsudaidai×unshiu) Judged from Isozyme Patterns in Organs and Tissues
Chimerism of Kobayashi-mikan (Citrus natsudaidai×unshiu) Judged from Isozyme Patterns in Organs and Tissues
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从器官和组织中的同工酶模式判断小林蜜柑（Citrus natsudaidai×unshiu）的嵌合现象
DOI：
10.2503/jjshs.52.223
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发表时间：
1983
影响因子：
--
通讯作者：
K. Yamashita
中科院分区：
文献类型：
--
作者：
K. Yamashita
本实验是为了证实关于小林-mikan嵌合的普遍公认的假设。该品种是由蜜桔（Citrus unshiu Marc.）接穗与夏水田（C. Natsudaidai Hayata）砧木杂交而成的合成嵌合体。用淀粉凝胶电泳法分析了叶片、果实部分、幼苗未成熟叶片和幼根的过氧化物酶和酯酶同工酶。对小林蜜柑、萨摩柑和那津台的酶谱分析结果总结如下。以7月份采集的样品为例，小林三馆新叶和老叶的过氧化物酶同工酶模式以及反照率与那津台的过氧化物酶同工酶模式明显相似。小林蜜柑汁囊的形态与萨摩柑十分相似。小林样黄酮和反照率的酯酶同工酶谱与那津台完全相同。在10月份采集的样品中，小林三馆的老叶和黄酮的过氧化物酶同工酶模式与那津台的几乎相同。小林宫的反照率和新叶的模式与夏秋台相似。小林苗老叶和种子的酯酶同工酶谱与那津台相同。小林三馆的黄酮类、肺泡和新叶的形态与那津台颇为相似。小林苗苗的幼叶和幼根的过氧化物酶和酯酶同工酶谱与那苏代苗的过氧化物酶和酯酶谱相似。这些结果进一步证实了小林三坎是萨摩柑与那津台之间移植物诱导的周嵌合体的假设。
The present experiment was undertaken to confirm the generally recognized assumption concerning the chimerism of Kobayashi-mikan. This cultivar is supposed to be a synthetic chimera which has arisen from the junction of satsuma mandarin (Citrus unshiu Marc.) scion and Natsudaidai (C. natsudaidai Hayata) stock. Peroxidase and esterase isozymes of foliage and fruit parts as well as immature leaves and rootlets of young seedlings were analyzed by starch gel electrophoresis. The results obtained from a zymographic comparison of Kobayashi-mikan, satsuma mandarin and Natsudaidai are summarized as follows.1. In the case of samples taken in July, peroxidase isozyme patterns of new and old leaves as well as albedo of Kobayashi-mikan distinctly resembled those of Natsudaidai. The pattern of juice sac of Kobayashi-mikan considerably resembled that of satsuma mandarin. Esterase isozyme patterns of flavedo and albedo of Kobayashi-mikan were completely the same as those of Natsudaidai.2. In the case of samples taken in October, peroxidase isozyme patterns of old leaves as well as flavedo of Kobayashi-mikan were nearly the same as those of Natsudaidai. The patterns of albedo and new leaves of Kobayashi-mikan resembled those of Natsudaidai. Esterase isozyme patterns of old leaves and seeds of Kobayashi -mikan were equal to those of Natsudaidai. The patterns of flavedo, alvedo and new leaves of Kobayashi-mikan were rather similar to those of Natsudaidai.3. Peroxidase and esterase isozyme patterns of immature leaves and rootlets of young seedlings grown from seeds of Kobayashi-mikan were similar to those of young seedlings grown from seeds of Natsudaidai. From these results, the hypothesis that Kobayashi-mikan is a graft-induced periclinal chimera between satsuma mandarin and Natsudaidai has been further confirmed.