Zeranol enhances the proliferation of pre-adipocytes in beef heifers.
Zeranol enhances the proliferation of pre-adipocytes in beef heifers.
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发表时间：
2009-12
影响因子：
2
通讯作者：
Weiping Ye;Pingping Xu;W. R. Threlfall;R. Jen;Hong Li;Shu-Hong Lin;Cheik-Ti Kuo;Young C. Lin
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文献类型：
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作者：
Weiping Ye;Pingping Xu;W. R. Threlfall;R. Jen;Hong Li;Shu-Hong Lin;Cheik-Ti Kuo;Young C. Lin
背景女性乳腺癌的高发病率和高死亡率是一个严重的问题。玉米赤霉醇（Zeranol，一种在北美牛肉工业中广泛使用的生长促进剂）残留对人体健康的不良影响尚不清楚。材料与方法观察Z植入对小母牛前脂肪细胞生长的影响。测定了Z和雌二醇-17 β（E（2））对分离自对照小母牛和Z植入小母牛的前脂肪细胞增殖的刺激作用。采用实时荧光定量PCR和Western-blotting方法检测cyclin D1和p53在mRNA和蛋白水平的表达。结果：从承受2个月Z-植入物的小母牛中获得的前脂肪细胞的生长比在对照小母牛中观察到的快约12倍。从Z植入小母牛中分离的前脂肪细胞对Z和E处理更敏感（2）。Z上调细胞周期蛋白D1的表达和下调p53在前脂肪细胞分离Z植入小母牛。结论Z植入通过促进前脂肪细胞的生长而增加体重。Z和E（2）对前脂肪细胞分裂的刺激可能部分通过上调cyclin D1和下调p53在mRNA和蛋白水平上的表达而介导。
BACKGROUND The high morbidity and mortality of breast cancer among women is a serious problem. The adverse effects of the consumption of beef with zeranol (Z, a growth promoter widely used in beef industry in North American) residue on human health are still unknown. MATERIALS AND METHODS The effects of Z implantation on the growth of heifer pre-adipocytes were evaluated. The stimulatory effects of Z and estradiol-17beta (E(2)) on the proliferation of pre-adipocytes isolated from control heifers and Z-implanted heifers were measured. Real-time PCR and Western-blotting analysis were performed to evaluate the expression of cyclin D1 and p53 at both mRNA and protein levels. RESULTS The growth of pre-adipocytes from heifers bearing for 2 months of Z-implants was about 12-fold faster than that observed in control heifers. The pre-adipocytes isolated from Z-implanted heifers were more sensitive to treatment with Z and E(2). Z up-regulated the expression of cyclin D1 and down-regulated p53 in pre-adipocytes isolated from Z-implanted heifers. CONCLUSION The implantation of Z increases body weight gain by enhancing growth of pre-adipocytes. The stimulation of pre-adipocytes division by Z and E(2) might be partially mediated by up-regulation of cyclin D1 and down-regulation of p53 at mRNA and protein levels.