Flp recombinase promotes site-specific DNA recombination in embryonic stem cells and transgenic mice
Flp recombinase promotes site-specific DNA recombination in embryonic stem cells and transgenic mice
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DOI：
10.1073/pnas.93.12.6191
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发表时间：
1996-06-11
影响因子：
11.1
通讯作者：
Dymecki, SM
中科院分区：
文献类型：
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作者：
Dymecki, SM
由于PIP重组酶可以催化DNA在不同识别靶点之间的精确切除、整合、倒置或移位，因此正被开发为“体内”基因工程的工具。本研究证明，PIP重组酶可以有效地介导小鼠胚胎干细胞中的位点特异性切除重组，在分化胚胎癌细胞中，在转基因小鼠中，广泛的FLP表达与正常发育相容，这表明PIP可用于催化大多数细胞类型的重组。这些特性表明，FLP可以被利用来对小鼠基因组进行规定的改变。
Site-specific recombinases are being developed as tools for ''in vivo'' genetic engineering because they can catalyze precise excisions, integrations, inversions, or translocations of DNA between their distinct recognition target sites, Here it is demonstrated that Pip recombinase can effectively mediate site-specific excisional recombination in mouse embryonic stem cells, in differentiating embryonal carcinoma cells, and in transgenic mice, Broad Flp expression is compatible with normal development, suggesting that Pip can be used to catalyze recombination in most cell types. These properties indicate that Flp can be exploited to make prescribed alterations in the mouse genome.