Isolation of DNA after extraction of RNA To detect the presence of Borrelia burgdorferi and expression of host cellular genes from the same tissue sample.
Isolation of DNA after extraction of RNA To detect the presence of Borrelia burgdorferi and expression of host cellular genes from the same tissue sample.
复制标题
提取 RNA 后分离 DNA 检测同一组织样本中伯氏疏螺旋体的存在以及宿主细胞基因的表达。
DOI：
10.1128/jcm.37.6.2087-2089.1999
复制
发表时间：
1999
影响因子：
9.4
通讯作者：
Pachner,AR
中科院分区：
文献类型：
--
作者：
Amemiya,K;Schaefer,H;Pachner,AR
We are investigating the neuropathogenesis of Lyme disease caused byBorrelia burgdorferiin a nonhuman primate model. In the past, two separate pieces of tissue had to be used when both analyzing for the presence of the spirochete and examining the host response to infection. We have modified a procedure to purify DNA from the same sample after the extraction of RNA. The remaining material containing the DNA was precipitated, and residual organic reagent was removed prior to deproteinization and extraction of the DNA. This procedure now allows us to both assay for the presence of the Lyme microorganism and analyze the host response in the same tissue preparation.
