Comprehensive diversity analysis of viroids infecting grapevine in China and Japan
Comprehensive diversity analysis of viroids infecting grapevine in China and Japan
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DOI：
10.1016/j.virusres.2012.08.008
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发表时间：
2012-10-01
期刊：
影响因子：
5
通讯作者：
Li, Shifang
中科院分区：
文献类型：
--
作者：
Jiang, Dongmei;Sano, Teruo;Li, Shifang
迄今为止，已发现几种类病毒感染葡萄，包括啤酒花矮化类病毒（HpSVd）、柑橘裂皮类病毒（CEVd）、澳大利亚葡萄类病毒（AGVd）、葡萄藤黄色斑点类病毒-1（GYSVd-1）、葡萄藤黄色斑点类病毒-2（GYSVd-2）和一个暂定的新种，葡萄藤黄色斑点类病毒-3（GYSVd-3）。在这里，我们确定和分析的分布，遗传多样性和分子特性的类病毒感染在中国和日本栽培的葡萄藤，包括老葡萄。结果表明，我国存在5种已知的葡萄类病毒，其中3种（HpSVd、GYSVd-1和GYSVd-3）存在于日本。中国和日本葡萄老藤类病毒多样性的差异可能反映了两国葡萄栽培历史的不同。总的来说，中国以及伊朗和澳大利亚葡萄藤上的类病毒种类比其他国家更多样化。感染中国和日本葡萄藤的类病毒种群结构表现出种属依赖性;即，HpSVd在两国具有相似的种群结构，但GYSVd-1、GYSVd-2和AGVd在同一国家内也存在区域差异，但序列多样性在侵染葡萄藤的类病毒生物学中的作用（如致病性和进化）仍需进一步研究。皇冠版权所有（C）2012由Elsevier B. V.出版。保留所有权利。
To date, several viroid species have been shown to infect grapevine, including Hop stunt viroid (HpSVd), Citrus exocortis viroid (CEVd), Australian grapevine viroid (AGVd), Grapevine yellow speckle viroid-1 (GYSVd-1), Grapevine yellow speckle viroid-2 (GYSVd-2) and a tentative new species, Grapevine yellow speckle viroid-3 (GYSVd-3). Here, we identified and analyzed the distribution, genetic diversity, and molecular properties of viroids infecting grapevine cultivated in China and Japan, including old grapevines. The analysis showed that all the five known viroids and a tentative species GYSVd-3 infecting grapevine exist in China, and three of them (HpSVd, GYSVd-1 and GYSVd-3) exist in Japan. The contrast in diversity of viroid species in old grapevines from China and Japan may reflect different history of viticulture between the two countries. In general, the species of viroids infecting grapevine in China, as well as those in Iran and Australia, were more diverse than in the other countries. The population structure of viroids infecting grapevine in China and Japan showed species-dependency; i.e., HpSVd shared similar population structures in both countries, but GYSVd-1, GYSVd-2, and AGVd showed regional disparity even within the same country, although the role of sequence diversity in the biology of viroids infecting grapevine, such as the pathogenicity and evolution, still needs further study. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved.